PMID- 23045145
OWN - NLM
STAT- MEDLINE
DCOM- 20130308
LR  - 20220310
IS  - 1934-9262 (Electronic)
IS  - 1934-9254 (Linking)
VI  - Chapter 4
DP  - 2007
TI  - Measurement of soluble epoxide hydrolase (sEH) activity.
PG  - Unit 4.23
LID - 10.1002/0471140856.tx0423s33 [doi]
AB  - The human soluble epoxide hydrolase (sEH; EC 3.3.3.2) is the product of the EXPH2 
      gene. The sEH catalyzes the addition of a water molecule to an epoxide, resulting 
      in the corresponding diol. Early work suggested a role of sEH in detoxifying a 
      wide array of xenobiotic epoxides; however, recent findings clearly implicate the 
      sEH in the regulation of blood pressure, pain, and inflammation through the 
      hydrolysis of endogenous epoxy fatty acids such as epoxyeicosatrienoic acids 
      (EETs). Both expression and activity of sEH are influenced by a wide array of 
      xenobiotics, underlying how environmental contaminants could influence human 
      health through sEH. This unit describes radiometric, fluorimetric, and mass 
      spectrometric assays for measuring the activity of sEH and its inhibition.
FAU - Morisseau, Christophe
AU  - Morisseau C
AD  - University of California at Davis, Davis, California, USA.
FAU - Hammock, Bruce D
AU  - Hammock BD
LA  - eng
GR  - P30 ES05707/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699/ES/NIEHS NIH HHS/United States
GR  - R37 ES02710/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Curr Protoc Toxicol
JT  - Current protocols in toxicology
JID - 9816330
RN  - 0 (Environmental Pollutants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Small Molecule Libraries)
RN  - 0 (Xenobiotics)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Chromatography, Liquid
MH  - Environmental Pollutants/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Epoxide Hydrolases/antagonists & inhibitors/*metabolism
MH  - Epoxy Compounds/metabolism
MH  - Fluorometry
MH  - High-Throughput Screening Assays
MH  - Humans
MH  - Inactivation, Metabolic
MH  - Small Molecule Libraries/pharmacology
MH  - Solubility
MH  - Substrate Specificity
MH  - Tandem Mass Spectrometry
MH  - Xenobiotics/metabolism
EDAT- 2007/08/01 00:00
MHDA- 2013/03/09 06:00
CRDT- 2012/10/10 06:00
PHST- 2012/10/10 06:00 [entrez]
PHST- 2007/08/01 00:00 [pubmed]
PHST- 2013/03/09 06:00 [medline]
AID - 10.1002/0471140856.tx0423s33 [doi]
PST - ppublish
SO  - Curr Protoc Toxicol. 2007;Chapter 4:Unit 4.23. doi: 10.1002/0471140856.tx0423s33.