PMID- 23045950
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20121010
IS  - 0034-8376 (Print)
IS  - 0034-8376 (Linking)
VI  - 64
IP  - 3
DP  - 2012 May-Jun
TI  - The usefulness of the buffy coat smear and panbacterial polymerase chain reaction 
      in early diagnosis of neonatal sepsis.
PG  - 275-83
AB  - OBJECTIVE: In this study are evaluated the usefulness of the buffy coat smear and 
      panbacterial polymerase chain reaction (PCR) as diagnostic tests in the early 
      detection of neonatal sepsis. MATERIAL AND METHODS: It was studied 49 patients 
      aged up to 28 days who were hospitalized in the Intensive Care Unit (ICUs) of the 
      Neonatology, with a clinical diagnosis of neonatal sepsis and 49 umbilical cord 
      samples of healthy newborns. Blood cultures and 50 microL of plasma were taken 
      for the DNA and performance of the broad-range PCR primer system (panbacterial 
      PCR). Simultaneously, were taken three capillaries with blood for the leukocyte 
      layer (buffy coat) smear, we performed three stains: Gram; Loeffler blue 
      methylene (LBM), and acridine orange (AO). Statistical analysis included 
      sensitivity, specificity, positive predictive value (PPV), and negative 
      predictive value (NPV) against the clinical diagnosis. RESULTS: With respect to 
      stains of buffy coat smear, they resulted very specific, from 90-97%, with 64-75% 
      sensitivity, 87-94% PPV, and 77-82% NPV. In inverse fashion, PCR resulted very 
      sensitive at 96%, with 91% specificity, 92% PPV, and 96% NPV. CONCLUSIONS: Buffy 
      coat smear stains are easy, fast, and specific, while that of PCR was highly 
      sensitive. Thus, both can be utilized as diagnostic tests.
FAU - Garcia-Elorriaga, Guadalupe
AU  - Garcia-Elorriaga G
AD  - Unidad de Investigaci6n en Inmunologia e Infectologia, Centro Medico Nacional La 
      Raza, Instituto Mexicano del Seguro Social, Mexico, D.F. 
      gelorriaga@webtelmex.net.mx
FAU - Cortes-Torres, Nancy
AU  - Cortes-Torres N
FAU - Ballesteros-Del-Olmo, Julio Cesar
AU  - Ballesteros-Del-Olmo JC
FAU - Del Rey-Pineda, Guillermo
AU  - Del Rey-Pineda G
FAU - Gonzaez-Bonilla, Cesar
AU  - Gonzaez-Bonilla C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Mexico
TA  - Rev Invest Clin
JT  - Revista de investigacion clinica; organo del Hospital de Enfermedades de la 
      Nutricion
JID - 9421552
SB  - IM
MH  - Bacteriological Techniques/methods
MH  - Early Diagnosis
MH  - Female
MH  - Humans
MH  - Infant, Newborn
MH  - Male
MH  - Polymerase Chain Reaction/*methods
MH  - Sepsis/*blood/*diagnosis
MH  - Staining and Labeling
EDAT- 2012/10/11 06:00
MHDA- 2012/11/02 06:00
CRDT- 2012/10/11 06:00
PHST- 2012/10/11 06:00 [entrez]
PHST- 2012/10/11 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
PST - ppublish
SO  - Rev Invest Clin. 2012 May-Jun;64(3):275-83.