PMID- 23047205
OWN - NLM
STAT- MEDLINE
DCOM- 20130318
LR  - 20121010
IS  - 1658-3175 (Electronic)
IS  - 0379-5284 (Linking)
VI  - 33
IP  - 10
DP  - 2012 Oct
TI  - Successful pregnancies after combined human leukocyte antigen direct genotyping 
      and preimplantation genetic diagnosis utilizing multiple displacement 
      amplification.
PG  - 1059-64
AB  - OBJECTIVE: To devise a new and simple technique to help select normal embryos 
      that are human leukocyte antigen (HLA) matched to their affected siblings for 
      diseases, such as beta-thalassemia or sickle cell anemia, which are common in 
      this part of the world. METHODS: This study was conducted between March 2008 and 
      April 2011 at the preimplantation Genetic Diagnosis Laboratory, Saad Specialist 
      Hospital, Al-Khobar, Kingdom of Saudi Arabia. Embryos were obtained after 7 in 
      vitro fertilization preimplantation genetic diagnosis (IVF-PGD) cycles. Single 
      cells were biopsied, and extracted DNA was amplified by the multiple displacement 
      amplification (MDA) technique. Amplified DNA was then tested for mutations in the 
      beta-globin gene, and directly HLA typed using a sequence specific primer 
      technique. RESULTS: We report 7 families that underwent 7 PGD cycles with HLA 
      typing and direct HLA loci-typing using an HLA conventional commercial kit. Two 
      patients had PGD and HLA typing for class I and II, while the other 5 had class 
      II. The PGD cycles resulted in 3 pregnancies out of 5 patients who had HLA 
      matched and normal embryos. One family had a successful hematopoietic stem cell 
      transplant. CONCLUSION: This report demonstrates the first clinical application 
      of PGD coupled with direct HLA loci-typing of DNA amplified by MDA from a single 
      cell.
FAU - Hellani, Ali M
AU  - Hellani AM
AD  - Genetic Diagnosis Laboratory, Saad Specialist Hospital, Al-Khobar, Kingdom of 
      Saudi Arabia.
FAU - Akoum, Siham M
AU  - Akoum SM
FAU - Fadel, Elias S
AU  - Fadel ES
FAU - Yousef, Hisham M
AU  - Yousef HM
FAU - Abu-Amero, Khaled K
AU  - Abu-Amero KK
LA  - eng
PT  - Journal Article
PL  - Saudi Arabia
TA  - Saudi Med J
JT  - Saudi medical journal
JID - 7909441
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Female
MH  - Genotype
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
EDAT- 2012/10/11 06:00
MHDA- 2013/03/19 06:00
CRDT- 2012/10/11 06:00
PHST- 2012/10/11 06:00 [entrez]
PHST- 2012/10/11 06:00 [pubmed]
PHST- 2013/03/19 06:00 [medline]
AID - 0' [pii]
PST - ppublish
SO  - Saudi Med J. 2012 Oct;33(10):1059-64.