PMID- 23047828
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20221207
IS  - 1439-4286 (Electronic)
IS  - 0018-5043 (Linking)
VI  - 45
IP  - 2
DP  - 2013 Feb
TI  - Circulating very-low-density lipoprotein from subjects with impaired glucose 
      tolerance accelerates adrenocortical cortisol and aldosterone synthesis.
PG  - 169-72
LID - 10.1055/s-0032-1323844 [doi]
AB  - Apart from their role in cardiovascular homeostasis and immunomodulation, 
      aldosterone and cortisol are also implicated in the pathogenesis of insulin 
      resistance and type 2 diabetes mellitus (T2DM). Furthermore, glycoxidative 
      modifications of lipoproteins are increasingly recognized as an etiological 
      factor for increased cardiovascular morbidity and mortality in prediabetic 
      individuals. The causative relationship between in vivo lipoprotein modifications 
      and steroidogenesis in subjects with impaired glucose tolerance (IGT), however, 
      is not well defined. Therefore, we aimed to investigate the impact of in vivo 
      modified lipoproteins on aldosterone and cortisol release from human 
      adrenocortical H295R cells. Following an oral glucose tolerance test, 20 
      individuals with normal glucose tolerance (NGT) and 20 IGT subjects were randomly 
      selected from the ongoing PRAEDIAS prevention study in our department. Cells were 
      incubated for 24 h with lipoproteins isolated from NGT and IGT individuals and 
      aldosterone and cortisol release was measured in the supernatants. VLDL induced a 
      greater stimulating effect on adrenocortical aldosterone and cortisol release 
      compared to HDL and LDL. Moreover, IGT-VLDL evoked a significantly higher effect 
      (p<0.05) on hormone release than NGT-VLDL. Incubation of cells with in vitro 
      modified lipoproteins and specific pharmacological inhibitors suggests that VLDL 
      presumably recruits ERK1/2 as one of the downstream effectors of Jak-2. In 
      summary, in vivo modified VLDL are able to promote prediabetic hormonal 
      dysregulation by modulating adrenocortical steroidogenesis via Jak-2-ERK 
      dependent pathway.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Saha, S
AU  - Saha S
AD  - Department of Internal Medicine 3, Carl Gustav Carus Medical School, Technical 
      University of Dresden, Dresden, Germany.
FAU - Schwarz, P E H
AU  - Schwarz PE
FAU - Bergmann, S
AU  - Bergmann S
FAU - Bornstein, S R
AU  - Bornstein SR
FAU - Graessler, J
AU  - Graessler J
FAU - Kopprasch, S
AU  - Kopprasch S
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121009
PL  - Germany
TA  - Horm Metab Res
JT  - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et 
      metabolisme
JID - 0177722
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (hemoglobin A1c protein, human)
RN  - 4964P6T9RB (Aldosterone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adrenal Cortex/drug effects/*metabolism
MH  - Aged
MH  - Aldosterone/*metabolism
MH  - Cell Line
MH  - Glucose Intolerance/blood/*metabolism/physiopathology
MH  - Glycated Hemoglobin/analysis
MH  - Humans
MH  - Hydrocortisone/*metabolism
MH  - Hyperglycemia/etiology
MH  - Lipoproteins, HDL/blood/metabolism
MH  - Lipoproteins, LDL/blood/metabolism
MH  - Lipoproteins, VLDL/blood/*metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Male
MH  - Middle Aged
MH  - Phosphorylation/drug effects
MH  - Prediabetic State/blood/metabolism/physiopathology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Protein Processing, Post-Translational/drug effects
MH  - *Up-Regulation/drug effects
EDAT- 2012/10/11 06:00
MHDA- 2013/09/04 06:00
CRDT- 2012/10/11 06:00
PHST- 2012/10/11 06:00 [entrez]
PHST- 2012/10/11 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - 10.1055/s-0032-1323844 [doi]
PST - ppublish
SO  - Horm Metab Res. 2013 Feb;45(2):169-72. doi: 10.1055/s-0032-1323844. Epub 2012 Oct 
      9.