PMID- 23049171
OWN - NLM
STAT- MEDLINE
DCOM- 20130204
LR  - 20211021
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2012
DP  - 2012
TI  - MCP-1-induced histamine release from mast cells is associated with development of 
      interstitial cystitis/bladder pain syndrome in rat models.
PG  - 358184
LID - 10.1155/2012/358184 [doi]
LID - 358184
AB  - OBJECTIVE: Interstitial cystitis/bladder pain syndrome (IC/BPS) is characterized 
      by overexpression of monocyte chemoattractant protein-1 (MCP-1) in bladder 
      tissues and induction of mast cell (MC) degranulation. This study was undertaken 
      to explore the mechanism of action of MCP-1 in the development of IC/BPS. 
      METHODS: A rat model of IC/BPS was developed by perfusing bladders of nine SPF- 
      grade female Sprague-Dawley rats with protamine sulfate and lipopolysaccharide 
      (PS+LPS). MCP-1 and histamine levels in bladder tissue and urine were detected by 
      immunohistochemistry and ELISA. MC degranulation was measured by 
      immunofluorescence techniques and chemokine (C-C motif) receptor 2 (CCR2) was 
      assayed by flow cytometry. RESULTS: Increased MCP-1 expression in bladder tissue 
      and elevated MCP-1 and histamine levels were observed in the urine of 
      LS+LPS-treated rats. This was accompanied by the expression of CCR2 on MC 
      surfaces, suggesting MCP-1 may induce MC degranulation through CCR2. Exposure to 
      LPS stimulated MCP-1 expression in bladder epithelial cells, and exposure to 
      MCP-1 induced histamine release from MCs. CONCLUSIONS: MCP-1 upregulation in 
      IC/BPS is one of possible contributing factors inducing histamine release from 
      MCs. CCR2 is involved in the process of mast cell degranulation in bladder 
      tissues. These changes may contribute to the development of symptoms of IC/BPS.
FAU - Lv, Jianwei
AU  - Lv J
AD  - Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai 200127, China.
FAU - Huang, Yiran
AU  - Huang Y
FAU - Zhu, Shiguo
AU  - Zhu S
FAU - Yang, Ganggang
AU  - Yang G
FAU - Zhang, Yujian
AU  - Zhang Y
FAU - Leng, Jing
AU  - Leng J
FAU - Bo, Juanjie
AU  - Bo J
FAU - Liu, Dongming
AU  - Liu D
LA  - eng
PT  - Journal Article
DEP - 20120919
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, CCR2)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism
MH  - Cystitis, Interstitial/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Histamine/metabolism
MH  - Histamine Release/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lipopolysaccharides/pharmacology
MH  - Mast Cells/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CCR2/metabolism
PMC - PMC3459284
EDAT- 2012/10/11 06:00
MHDA- 2013/02/05 06:00
PMCR- 2012/09/19
CRDT- 2012/10/11 06:00
PHST- 2012/02/29 00:00 [received]
PHST- 2012/06/08 00:00 [revised]
PHST- 2012/06/22 00:00 [accepted]
PHST- 2012/10/11 06:00 [entrez]
PHST- 2012/10/11 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
PHST- 2012/09/19 00:00 [pmc-release]
AID - 10.1155/2012/358184 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2012;2012:358184. doi: 10.1155/2012/358184. Epub 2012 Sep 19.