PMID- 23049780
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20240313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 9
DP  - 2012
TI  - Exploiting the combination of natural and genetically engineered resistance to 
      cassava mosaic and cassava brown streak viruses impacting cassava production in 
      Africa.
PG  - e45277
LID - 10.1371/journal.pone.0045277 [doi]
LID - e45277
AB  - Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are 
      currently two major viral diseases that severely reduce cassava production in 
      large areas of Sub-Saharan Africa. Natural resistance has so far only been 
      reported for CMD in cassava. CBSD is caused by two virus species, Cassava brown 
      streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). A sequence of 
      the CBSV coat protein (CP) highly conserved between the two virus species was 
      used to demonstrate that a CBSV-CP hairpin construct sufficed to generate 
      immunity against both viral species in the cassava model cultivar (cv. 60444). 
      Most of the transgenic lines showed high levels of resistance under increasing 
      viral loads using a stringent top-grafting method of inoculation. No viral 
      replication was observed in the resistant transgenic lines and they remained free 
      of typical CBSD root symptoms 7 month post-infection. To generate transgenic 
      cassava lines combining resistance to both CBSD and CMD the hairpin construct was 
      transferred to a CMD-resistant farmer-preferred Nigerian landrace TME 7 
      (Oko-Iyawo). An adapted protocol allowed the efficient Agrobacterium-based 
      transformation of TME 7 and the regeneration of transgenic lines with high levels 
      of CBSV-CP hairpin-derived small RNAs. All transgenic TME 7 lines were immune to 
      both CBSV and UCBSV infections. Further evaluation of the transgenic TME 7 lines 
      revealed that CBSD resistance was maintained when plants were co-inoculated with 
      East African cassava mosaic virus (EACMV), a geminivirus causing CMD. The 
      innovative combination of natural and engineered virus resistance in 
      farmer-preferred landraces will be particularly important to reducing the 
      increasing impact of cassava viral diseases in Africa.
FAU - Vanderschuren, Herve
AU  - Vanderschuren H
AD  - Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland. 
      hvanderschuren@ethz.ch
FAU - Moreno, Isabel
AU  - Moreno I
FAU - Anjanappa, Ravi B
AU  - Anjanappa RB
FAU - Zainuddin, Ima M
AU  - Zainuddin IM
FAU - Gruissem, Wilhelm
AU  - Gruissem W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120925
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Africa South of the Sahara
MH  - Agrobacterium/genetics
MH  - Begomovirus/pathogenicity/*physiology
MH  - Genetic Engineering
MH  - Inverted Repeat Sequences/genetics
MH  - Manihot/genetics/*immunology/virology
MH  - Plant Diseases/genetics/*immunology/virology
MH  - Plant Immunity/genetics
MH  - *Plants, Genetically Modified
MH  - Potyviridae/pathogenicity/*physiology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - RNA, Viral/*genetics
MH  - Sequence Analysis, DNA
MH  - Transformation, Genetic
MH  - Viral Proteins/chemistry/*genetics
PMC - PMC3458115
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/10/11 06:00
MHDA- 2013/04/23 06:00
PMCR- 2012/09/25
CRDT- 2012/10/11 06:00
PHST- 2012/05/17 00:00 [received]
PHST- 2012/08/15 00:00 [accepted]
PHST- 2012/10/11 06:00 [entrez]
PHST- 2012/10/11 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
PHST- 2012/09/25 00:00 [pmc-release]
AID - PONE-D-12-14443 [pii]
AID - 10.1371/journal.pone.0045277 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(9):e45277. doi: 10.1371/journal.pone.0045277. Epub 2012 Sep 25.