PMID- 23050672
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20161125
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 25
IP  - 7
DP  - 2013
TI  - Differential expression of the P2X7 receptor in ovarian surface epithelium during 
      the oestrous cycle in the mouse.
PG  - 971-84
LID - 10.1071/RD12196 [doi]
AB  - Purinergic signalling has been proposed as an intraovarian regulatory mechanism. 
      Of the receptors responsible for purinergic transmission, the P2X7 receptor is an 
      ATP-gated cationic channel that displays a broad spectrum of cellular functions 
      ranging from apoptosis to cell proliferation and tumourigenesis. In the present 
      study, we investigated the functional expression of P2X7 receptors in ovarian 
      surface epithelium (OSE). P2X7 protein was detected in the OSE layer of the 
      mouse, both in situ and in primary cultures. In cultures, 
      2'(3')-O-(4-Benzoylbenzoyl)adenosine-5'-triphosphate (BzATP) activation of P2X7 
      receptors increased [Ca(2+)]i and induced apoptosis. The functionality of the 
      P2X7 receptor was investigated in situ by intrabursal injection of BzATP on each 
      day of the oestrous cycle and evaluation of apoptosis 24h using the terminal 
      deoxyribonucleotidyl transferase-mediated dUTP-fluorescein nick end-labelling 
      (TUNEL) assay. Maximum effects of BzATP were observed during pro-oestrus, with 
      the effects being blocked by A438079, a specific P2X7 receptor antagonist. 
      Immunofluorescence staining for P2X7 protein revealed more robust expression 
      during pro-oestrus and in OSE regions behind the antral follicles, strongly 
      supporting the notion that the differences in apoptosis can be explained by 
      increased receptor expression, which is regulated during the oestrous cycle. 
      Finally, P2X7 receptor expression was detected in the OSE layer of human ovaries, 
      with receptor expression maintained in human ovaries diagnosed with cancer, as 
      well as in the human ovarian carcinoma SKOV3 cell line.
FAU - Vazquez-Cuevas, F G
AU  - Vazquez-Cuevas FG
AD  - Departamento de Neurobiologia Celular y Molecular, Instituto de Neurobiologia, 
      Universidad Nacional Autonoma de Mexico, Boulevard Juriquilla 3001, Juriquilla 
      Queretaro, CP, 76230, Queretaro Mexico.
FAU - Cruz-Rico, A
AU  - Cruz-Rico A
FAU - Garay, E
AU  - Garay E
FAU - Garcia-Carranca, A
AU  - Garcia-Carranca A
FAU - Perez-Montiel, D
AU  - Perez-Montiel D
FAU - Juarez, B
AU  - Juarez B
FAU - Arellano, R O
AU  - Arellano RO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (3-(5-(2,3-dichlorophenyl)-1H-tetrazol-1-yl)methylpyridine)
RN  - 0 (Purinergic P2X Receptor Agonists)
RN  - 0 (Purinergic P2X Receptor Antagonists)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 0 (Tetrazoles)
RN  - 4P5DXU1F8Q (3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/analogs & derivatives/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Calcium/metabolism
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Epithelium/chemistry/physiology
MH  - Estrous Cycle/*physiology
MH  - Female
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Mice
MH  - Ovarian Neoplasms/chemistry
MH  - Ovary/*chemistry/physiology
MH  - Purinergic P2X Receptor Agonists/pharmacology
MH  - Purinergic P2X Receptor Antagonists/pharmacology
MH  - Pyridines/pharmacology
MH  - Receptors, Purinergic P2X7/*analysis/drug effects/*physiology
MH  - Tetrazoles/pharmacology
EDAT- 2012/10/12 06:00
MHDA- 2014/03/13 06:00
CRDT- 2012/10/12 06:00
PHST- 2012/06/21 00:00 [received]
PHST- 2012/09/04 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - RD12196 [pii]
AID - 10.1071/RD12196 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2013;25(7):971-84. doi: 10.1071/RD12196.