PMID- 23050791
OWN - NLM
STAT- MEDLINE
DCOM- 20130425
LR  - 20211021
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Print)
IS  - 0028-646X (Linking)
VI  - 196
IP  - 4
DP  - 2012 Dec
TI  - Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and 
      enhanced homologous recombination in rice.
PG  - 1048-1059
LID - 10.1111/j.1469-8137.2012.04350.x [doi]
AB  - Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in 
      Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the 
      model plant Arabidopsis remains inconclusive. Having established a rapid and 
      highly efficient Agrobacterium-mediated transformation system in rice (Oryza 
      sativa) using scutellum-derived calli, we examined here the involvement of the 
      NHEJ pathway in Agrobacterium-mediated stable transformation in rice. Rice calli 
      from OsKu70, OsKu80 and OsLig4 knockdown (KD) plants were infected with 
      Agrobacterium harboring a sensitive emerald luciferase (LUC) reporter construct 
      to evaluate stable expression and a green fluorescent protein (GFP) construct to 
      monitor transient expression of T-DNA. Transient expression was not suppressed, 
      but stable expression was reduced significantly, in KD plants. Furthermore, 
      KD-Ku70 and KD-Lig4 calli exhibited an increase in the frequency of homologous 
      recombination (HR) compared with control calli. In addition, suppression of 
      OsKu70, OsKu80 and OsLig4 induced the expression of HR-related genes on treatment 
      with DNA-damaging agents. Our findings suggest strongly that NHEJ is involved in 
      Agrobacterium-mediated stable transformation in rice, and that there is a 
      competitive and complementary relationship between the NHEJ and HR pathways for 
      DNA double-strand break repair in rice.
CI  - (c) 2012 The Authors. New Phytologist (c) 2012 New Phytologist Trust.
FAU - Nishizawa-Yokoi, Ayako
AU  - Nishizawa-Yokoi A
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
FAU - Nonaka, Satoko
AU  - Nonaka S
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 
      Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
FAU - Saika, Hiroaki
AU  - Saika H
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
FAU - Kwon, Yong-Ik
AU  - Kwon YI
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
AD  - Kihara Institute for Biological Research, Yokohama City University, 641-12 
      Maioka-cho, Yokohama, 244-0813, Japan.
FAU - Osakabe, Keishi
AU  - Osakabe K
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
AD  - Institute for Environmental Science and Technology, Saitama University, 
      Shimo-Okubo 255, Sakura-ku, Saitama-shi, 338-8570, Japan.
FAU - Toki, Seiichi
AU  - Toki S
AD  - Plant Genome Engineering Research Unit, National Institute of Agrobiological 
      Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
AD  - Kihara Institute for Biological Research, Yokohama City University, 641-12 
      Maioka-cho, Yokohama, 244-0813, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121010
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (T-DNA)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 6.5.1.- (DNA Ligases)
RN  - EC 6.5.1.1 (DNA Ligase ATP)
SB  - IM
MH  - Agrobacterium/genetics
MH  - DNA Breaks, Double-Stranded
MH  - DNA End-Joining Repair/genetics
MH  - DNA Helicases/*genetics/metabolism
MH  - DNA Ligase ATP
MH  - DNA Ligases/genetics/metabolism
MH  - DNA Repair
MH  - DNA, Bacterial
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Gene Knockdown Techniques
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - *Homologous Recombination
MH  - Oryza/*genetics/metabolism
MH  - Plant Proteins/*genetics/metabolism
MH  - Plants, Genetically Modified
MH  - *Transformation, Genetic
PMC - PMC3532656
EDAT- 2012/10/12 06:00
MHDA- 2013/04/26 06:00
CRDT- 2012/10/12 06:00
PHST- 2012/07/03 00:00 [received]
PHST- 2012/08/23 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2013/04/26 06:00 [medline]
AID - 10.1111/j.1469-8137.2012.04350.x [doi]
PST - ppublish
SO  - New Phytol. 2012 Dec;196(4):1048-1059. doi: 10.1111/j.1469-8137.2012.04350.x. 
      Epub 2012 Oct 10.