PMID- 23051709
OWN - NLM
STAT- MEDLINE
DCOM- 20130117
LR  - 20121030
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 434
IP  - 1
DP  - 2012 Dec 5
TI  - DNA shuffling of the GP3 genes of porcine reproductive and respiratory syndrome 
      virus (PRRSV) produces a chimeric virus with an improved cross-neutralizing 
      ability against a heterologous PRRSV strain.
PG  - 96-109
LID - S0042-6822(12)00446-1 [pii]
LID - 10.1016/j.virol.2012.09.005 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine 
      pathogen. Here we applied the DNA shuffling approaches to molecularly breed the 
      PRRSV GP3 gene, a neutralizing antibodies inducer, in an attempt to improve its 
      heterologous cross-neutralizing ability. The GP3 genes of six different PRRSV 
      strains were bred by traditional DNA shuffling. Additionally, synthetic DNA 
      shuffling of the GP3 gene was also performed using degenerate oligonucleotides. 
      The shuffled-GP3-libraries were cloned into the backbone of a DNA-launched PRRSV 
      infectious clone pIR-VR2385-CA. Four traditional-shuffled chimeras each 
      representing all 6 parental strains and four other synthetic-shuffled chimeras 
      were successfully rescued. These chimeras displayed similar levels of replication 
      both in vitro and in vivo, compared to the backbone parental virus, indicating 
      that the GP3 shuffling did not impair the replication capability of the chimeras. 
      One chimera GP3TS22 induced significantly higher levels of cross-neutralizing 
      antibodies in pigs against a heterologous PRRSV strain FL-12.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University (Virginia Tech), 
      Blacksburg, VA 24061-0913, USA.
FAU - Ni, Yan-Yan
AU  - Ni YY
FAU - Pineyro, Pablo
AU  - Pineyro P
FAU - Sanford, Brenton J
AU  - Sanford BJ
FAU - Cossaboom, Caitlin M
AU  - Cossaboom CM
FAU - Dryman, Barbara A
AU  - Dryman BA
FAU - Huang, Yao-Wei
AU  - Huang YW
FAU - Cao, Dian-Jun
AU  - Cao DJ
FAU - Meng, Xiang-Jin
AU  - Meng XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121007
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Neutralizing/*immunology
MH  - Antibodies, Viral/*immunology
MH  - Base Sequence
MH  - Cell Line
MH  - Cross Reactions
MH  - *DNA Shuffling
MH  - Disease Models, Animal
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*immunology/physiology
MH  - Swine
MH  - Viral Load
MH  - Viral Structural Proteins/*drug effects/*immunology
MH  - Virus Replication
EDAT- 2012/10/12 06:00
MHDA- 2013/01/18 06:00
CRDT- 2012/10/12 06:00
PHST- 2012/07/16 00:00 [received]
PHST- 2012/08/30 00:00 [revised]
PHST- 2012/09/10 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2013/01/18 06:00 [medline]
AID - S0042-6822(12)00446-1 [pii]
AID - 10.1016/j.virol.2012.09.005 [doi]
PST - ppublish
SO  - Virology. 2012 Dec 5;434(1):96-109. doi: 10.1016/j.virol.2012.09.005. Epub 2012 
      Oct 7.