PMID- 23053088
OWN - NLM
STAT- MEDLINE
DCOM- 20140108
LR  - 20130701
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 97
IP  - 14
DP  - 2013 Jul
TI  - Nitrifying bacterial community structures and their nitrification performance 
      under sufficient and limited inorganic carbon conditions.
PG  - 6513-23
LID - 10.1007/s00253-012-4436-y [doi]
AB  - This study examined the hypothesis that different inorganic carbon (IC) 
      conditions enrich different ammonia-oxidizing bacteria (AOB) and 
      nitrite-oxidizing bacteria (NOB) populations by operating two laboratory-scale 
      continuous-flow bioreactors fed with 15 and 100 mg IC/L, respectively. During 
      this study, both bioreactors maintained satisfactory nitrification performance 
      and stably oxidized 250 mg N/L of influent ammonium without nitrite accumulation. 
      Based on results of cloning/sequencing and terminal restriction fragment length 
      polymorphism targeting on the ammonia monooxygenase subunit A (amoA) gene, 
      Nitrosomonas nitrosa lineage was identified as the dominant AOB population in the 
      high-IC bioreactor, while Nitrosomonas europaea and Nitrosomonas nitrosa lineage 
      AOB were dominant in the low-IC bioreactor. Results of real-time polymerase chain 
      reactions for Nitrobacter and Nitrospira 16S rRNA genes indicated that Nitrospira 
      was the predominant NOB population in the high-IC bioreactor, while Nitrobacter 
      was the dominant NOB in the low-IC bioreactor. Furthermore, batch experiment 
      results suggest that N. europaea and Nitrobacter populations are proliferated in 
      the low-IC bioreactor due to their higher rates under low IC conditions despite 
      the fact that these two populations have been identified as weak competitors, 
      compared with N. nitrosa and Nitrospira, under low ammonium/nitrite environments. 
      This study revealed that in addition to ammonium/nitrite concentrations, limited 
      IC conditions may also be important in selecting dominant AOB/NOB communities of 
      nitrifying bioreactors.
FAU - Fukushima, Toshikazu
AU  - Fukushima T
AD  - Department of Environmental Engineering, National Cheng Kung University, Tainan 
      701, Taiwan, Republic of China.
FAU - Whang, Liang-Ming
AU  - Whang LM
FAU - Chiang, Ting-Yu
AU  - Chiang TY
FAU - Lin, Yi-Hsuan
AU  - Lin YH
FAU - Chevalier, Lizette R
AU  - Chevalier LR
FAU - Chen, Mei-Chun
AU  - Chen MC
FAU - Wu, Yi-Ju
AU  - Wu YJ
LA  - eng
SI  - GENBANK/AB622973
SI  - GENBANK/AB622974
SI  - GENBANK/AB622975
SI  - GENBANK/AB622976
SI  - GENBANK/AB622977
SI  - GENBANK/AB622978
SI  - GENBANK/AB622979
SI  - GENBANK/AB622980
SI  - GENBANK/AB622981
SI  - GENBANK/AB622982
SI  - GENBANK/AB622983
SI  - GENBANK/AB738418
SI  - GENBANK/AB738419
SI  - GENBANK/AB740393
SI  - GENBANK/AB740394
SI  - GENBANK/AB740395
SI  - GENBANK/AB740396
SI  - GENBANK/AB740397
SI  - GENBANK/AB740398
SI  - GENBANK/AB740399
SI  - GENBANK/AB740400
SI  - GENBANK/AB740401
SI  - GENBANK/AB740402
SI  - GENBANK/AB740403
SI  - GENBANK/AB740404
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121004
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Nitrites)
RN  - 0 (Sewage)
RN  - 7440-44-0 (Carbon)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Bacteria/classification/genetics/*isolation & purification/*metabolism
MH  - Bioreactors/microbiology
MH  - Carbon/*metabolism
MH  - Molecular Sequence Data
MH  - Nitrification
MH  - Nitrites/*metabolism
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Sewage/microbiology
EDAT- 2012/10/12 06:00
MHDA- 2014/01/09 06:00
CRDT- 2012/10/12 06:00
PHST- 2012/06/24 00:00 [received]
PHST- 2012/09/11 00:00 [accepted]
PHST- 2012/08/13 00:00 [revised]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2014/01/09 06:00 [medline]
AID - 10.1007/s00253-012-4436-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2013 Jul;97(14):6513-23. doi: 
      10.1007/s00253-012-4436-y. Epub 2012 Oct 4.