PMID- 23054819
OWN - NLM
STAT- MEDLINE
DCOM- 20130516
LR  - 20171116
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 168
IP  - 7
DP  - 2012 Dec
TI  - Two-stage cultivation of Pseudomonas sp. F12 for the production of enzymes 
      converting DL-2-amino-Delta(2)-thiazoline-4-carboxylic acid to L-cysteine.
PG  - 1867-79
LID - 10.1007/s12010-012-9903-5 [doi]
AB  - Pseudomonas sp. F12 isolated from soil could transform 
      DL-2-amino-Delta(2)-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine. It could 
      grow in minimal medium containing DL-ATC as the sole carbon and nitrogen source, 
      and the apparent activity of L-cysteine synthesis (CS) achieved 122 U/mL in a 5-L 
      bioreactor. Pseudomonas sp. F12 could utilize glucose as carbon source and 
      ammonia as nitrogen source for growth, but no CS activity was formed. To reduce 
      the cost of DL-ATC, the cultivation process was divided into a growth stage on 
      glucose and ammonia and a production stage induced by DL-ATC. The excessive 
      glucose led to the production of byproduct(s) which seriously inhibited cell 
      growth and CS production. Ammonium was accumulated when DL-ATC was consumed, and 
      ammonium did not inhibit CS activity formation until 60 mM. Based on the above 
      features, fed-batch cultivation of the growth stage was developed by supplying 
      glucose restrictively. The volumetric CS activity was enhanced more than two 
      times that obtained under the initial conditions.
FAU - Fan, Cuili
AU  - Fan C
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, China.
FAU - Li, Zhimin
AU  - Li Z
FAU - Ye, Qin
AU  - Ye Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121004
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Carboxylic Acids)
RN  - 0 (Culture Media)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Thiazoles)
RN  - 48431VN59G (2-amino-delta(2)-thiazoline-4-carboxylic acid)
RN  - EC 2.5.1.47 (Cysteine Synthase)
RN  - IY9XDZ35W2 (Glucose)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Batch Cell Culture Techniques/*methods
MH  - Biotransformation
MH  - Carboxylic Acids/*metabolism
MH  - Culture Media/chemistry
MH  - Cysteine/*metabolism
MH  - Cysteine Synthase/*biosynthesis/*metabolism
MH  - Glucose/metabolism
MH  - Pseudomonas/cytology/*enzymology/*growth & development/metabolism
MH  - Quaternary Ammonium Compounds/metabolism
MH  - Thiazoles/*metabolism
EDAT- 2012/10/12 06:00
MHDA- 2013/05/17 06:00
CRDT- 2012/10/12 06:00
PHST- 2012/05/27 00:00 [received]
PHST- 2012/09/18 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2013/05/17 06:00 [medline]
AID - 10.1007/s12010-012-9903-5 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2012 Dec;168(7):1867-79. doi: 10.1007/s12010-012-9903-5. 
      Epub 2012 Oct 4.