PMID- 23056274
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts 
      associated with Shinkaia crosnieri.
PG  - e46282
LID - 10.1371/journal.pone.0046282 [doi]
LID - e46282
AB  - In this study, we report experimental evidence of the thioautotrophic activity of 
      the epibiotic microbial community associated with the setae of Shinkaia 
      crosnieri, a galatheid crab that is endemic to deep-sea hydrothermal systems in 
      the Okinawa Trough in Japan. Microbial consumption of reduced sulfur compounds 
      under in situ hydrostatic and atmospheric pressure provided evidence of 
      sulfur-oxidizing activity by the epibiotic microbial community; the rate of 
      sulfur oxidation was similar under in situ and decompressed conditions. Results 
      of the microbial consumption of reduced sulfur compounds and tracer experiments 
      using (13)C-labeled bicarbonate in the presence and absence of thiosulfate (used 
      as a thioautotrophic substrate) convincingly demonstrated that the epibiotic 
      microbial community on S. crosnieri drove primary production via an energy 
      metabolism that was coupled with the oxidation of reductive sulfur compounds. A 
      combination of tracer experiments, fluorescence in situ hybridization (FISH) and 
      nano-scale secondary ion mass spectrometry (Nano-SIMS) indicated that the 
      filamentous cells of the genus Sulfurovum belonging to the class 
      Epsilonproteobacteria were thioautotrophs in the epibiotic community of S. 
      crosnieri. In conclusion, our results strongly suggest that thioautotrophic 
      production by Sulfurovum members present as the epibiotic microbial community 
      play a predominant role in a probable nutritional ectosymbiosis with S. 
      crosnieri.
FAU - Watsuji, Tomo-o
AU  - Watsuji TO
AD  - Subsurface Geobiology Advanced Research Team (SUGAR), Extremobiosphere Research 
      Program (XBR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science 
      and Technology (JAMSTEC), Yokosuka, Kanagawa, Japan. watsuji@jamstec.go.jp
FAU - Nishizawa, Manabu
AU  - Nishizawa M
FAU - Morono, Yuki
AU  - Morono Y
FAU - Hirayama, Hisako
AU  - Hirayama H
FAU - Kawagucci, Shinsuke
AU  - Kawagucci S
FAU - Takahata, Naoto
AU  - Takahata N
FAU - Sano, Yuji
AU  - Sano Y
FAU - Takai, Ken
AU  - Takai K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121002
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 70FD1KFU70 (Sulfur)
SB  - IM
MH  - Animals
MH  - Crustacea/*microbiology
MH  - Epsilonproteobacteria/genetics/metabolism/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Seawater/*microbiology
MH  - Spectrometry, Mass, Secondary Ion
MH  - Sulfur/metabolism
PMC - PMC3462759
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/10/12 06:00
MHDA- 2013/06/12 06:00
PMCR- 2012/10/02
CRDT- 2012/10/12 06:00
PHST- 2012/04/30 00:00 [received]
PHST- 2012/08/29 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
PHST- 2012/10/02 00:00 [pmc-release]
AID - PONE-D-12-13115 [pii]
AID - 10.1371/journal.pone.0046282 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e46282. doi: 10.1371/journal.pone.0046282. Epub 2012 Oct 2.