PMID- 23056631
OWN - NLM
STAT- MEDLINE
DCOM- 20130531
LR  - 20231105
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Neurally mediated airway constriction in human and other species: a comparative 
      study using precision-cut lung slices (PCLS).
PG  - e47344
LID - 10.1371/journal.pone.0047344 [doi]
LID - e47344
AB  - The peripheral airway innervation of the lower respiratory tract of mammals is 
      not completely functionally characterized. Recently, we have shown in rats that 
      precision-cut lung slices (PCLS) respond to electric field stimulation (EFS) and 
      provide a useful model to study neural airway responses in distal airways. Since 
      airway responses are known to exhibit considerable species differences, here we 
      examined the neural responses of PCLS prepared from mice, rats, guinea pigs, 
      sheep, marmosets and humans. Peripheral neurons were activated either by EFS or 
      by capsaicin. Bronchoconstriction in response to identical EFS conditions varied 
      between species in magnitude. Frequency response curves did reveal further 
      species-dependent differences of nerve activation in PCLS. Atropine antagonized 
      the EFS-induced bronchoconstriction in human, guinea pig, sheep, rat and marmoset 
      PCLS, showing cholinergic responses. Capsaicin (10 microM) caused bronchoconstriction 
      in human (4 from 7) and guinea pig lungs only, indicating excitatory 
      non-adrenergic non-cholinergic responses (eNANC). However, this effect was 
      notably smaller in human responder (30 +/- 7.1%) than in guinea pig (79 +/- 5.1%) 
      PCLS. The transient receptor potential (TRP) channel blockers SKF96365 and 
      ruthenium red antagonized airway contractions after exposure to EFS or capsaicin 
      in guinea pigs. In conclusion, the different species show distinct patterns of 
      nerve-mediated bronchoconstriction. In the most common experimental animals, i.e. 
      in mice and rats, these responses differ considerably from those in humans. On 
      the other hand, guinea pig and marmoset monkey mimic human responses well and may 
      thus serve as clinically relevant models to study neural airway responses.
FAU - Schleputz, Marco
AU  - Schleputz M
AD  - Institute of Pharmacology and Toxicology, RWTH Aachen University, Germany.
FAU - Rieg, Annette D
AU  - Rieg AD
FAU - Seehase, Sophie
AU  - Seehase S
FAU - Spillner, Jan
AU  - Spillner J
FAU - Perez-Bouza, Alberto
AU  - Perez-Bouza A
FAU - Braunschweig, Till
AU  - Braunschweig T
FAU - Schroeder, Thomas
AU  - Schroeder T
FAU - Bernau, Marc
AU  - Bernau M
FAU - Lambermont, Verena
AU  - Lambermont V
FAU - Schlumbohm, Christina
AU  - Schlumbohm C
FAU - Sewald, Katherina
AU  - Sewald K
FAU - Autschbach, Rudiger
AU  - Autschbach R
FAU - Braun, Armin
AU  - Braun A
FAU - Kramer, Boris W
AU  - Kramer BW
FAU - Uhlig, Stefan
AU  - Uhlig S
FAU - Martin, Christian
AU  - Martin C
LA  - eng
PT  - Journal Article
DEP - 20121009
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Imidazoles)
RN  - 11103-72-3 (Ruthenium Red)
RN  - I61V87164A (1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Bronchoconstriction/*drug effects
MH  - Calcium Channel Blockers/pharmacology
MH  - Callithrix
MH  - Capsaicin/pharmacology
MH  - Electric Stimulation
MH  - Guinea Pigs
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - In Vitro Techniques
MH  - Lung/*drug effects/*metabolism
MH  - Mice
MH  - Rats
MH  - Ruthenium Red/pharmacology
MH  - Sheep
PMC - PMC3467211
COIS- Competing Interests: CS is currently employee at Encepharm GmbH. With respect to 
      the current study CS took responsibility for the supply with NHP material from 
      the German Primate Center Gottingen, a non-commercial institution. There are no 
      competing interests by Encepharm GmbH relating to employment, consultancy, 
      patents, products in development or marketed products. Hence, CS's employment at 
      Encepharm GmbH does not alter the authors' adherence to all the PLOS ONE policies 
      on sharing data and materials.
EDAT- 2012/10/12 06:00
MHDA- 2013/06/01 06:00
PMCR- 2012/10/09
CRDT- 2012/10/12 06:00
PHST- 2012/04/20 00:00 [received]
PHST- 2012/09/11 00:00 [accepted]
PHST- 2012/10/12 06:00 [entrez]
PHST- 2012/10/12 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PHST- 2012/10/09 00:00 [pmc-release]
AID - PONE-D-12-11366 [pii]
AID - 10.1371/journal.pone.0047344 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e47344. doi: 10.1371/journal.pone.0047344. Epub 2012 Oct 9.