PMID- 23058476
OWN - NLM
STAT- MEDLINE
DCOM- 20130619
LR  - 20171116
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 61
IP  - 1
DP  - 2013 Jan
TI  - IL-15 activated human peripheral blood dendritic cell kill allogeneic and 
      xenogeneic endothelial cells via apoptosis.
PG  - 118-26
LID - S1043-4666(12)00681-3 [pii]
LID - 10.1016/j.cyto.2012.09.004 [doi]
AB  - IL-15 is a pleotropic cytokine, which plays an important role in natural killer 
      (NK) cell activity, T cell proliferation, and T cell cytotoxic activity. 
      Dendritic cells (DCs) are the major antigen presenting cells in the immune system 
      and presumed to play an important role in immune recognition of allo and 
      xenotransplantation. We showed that IL-15 activated human peripheral blood DC is 
      cytotoxic to human and porcine aortic endothelial cells. Unlike DCs, CD14+ 
      monocytes show no cytotoxicity against the endothelial cells. This cytotoxic 
      potential of IL-15 activated DC against endothelial cells is dose dependent and 
      increases significantly upon treatment of endothelial cells with inflammatory 
      cytokines like TNF-alpha or IFN-gamma. The cytotoxic potential of IL-15 activated DC is 
      associated with apoptosis of endothelial cells, as indicated by the increased 
      Annexin V staining, caspase activation and loss of mitochondrial membrane 
      potential. Further it was observed that DC mediated cytotoxicity against 
      endothelial cell is mediated via granzyme B possibly secreted by the activated 
      DCs.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Manna, Partha Pratim
AU  - Manna PP
AD  - Immunobiology Laboratory, Banaras Hindu University, Varanasi 221 005, India. 
      pp_manna@yahoo.com
FAU - Hira, Sumit Kumar
AU  - Hira SK
FAU - Das, Apabrita Ayan
AU  - Das AA
FAU - Bandyopadhyay, Santu
AU  - Bandyopadhyay S
FAU - Gupta, Kailash Kumar
AU  - Gupta KK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121009
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Interleukin-15)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.21.- (Granzymes)
SB  - IM
MH  - Animals
MH  - Aorta/immunology
MH  - Apoptosis/*immunology
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic/immunology
MH  - Dendritic Cells/*immunology
MH  - Endothelial Cells/*immunology
MH  - Granzymes/metabolism
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-15/*immunology/metabolism
MH  - Killer Cells, Natural/immunology
MH  - Lipopolysaccharide Receptors
MH  - Lymphocyte Activation/immunology
MH  - Membrane Potential, Mitochondrial
MH  - Monocytes/metabolism
MH  - Swine
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Transplantation, Heterologous/immunology
MH  - Transplantation, Homologous/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2012/10/13 06:00
MHDA- 2013/06/20 06:00
CRDT- 2012/10/13 06:00
PHST- 2012/04/20 00:00 [received]
PHST- 2012/07/15 00:00 [revised]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/10/13 06:00 [entrez]
PHST- 2012/10/13 06:00 [pubmed]
PHST- 2013/06/20 06:00 [medline]
AID - S1043-4666(12)00681-3 [pii]
AID - 10.1016/j.cyto.2012.09.004 [doi]
PST - ppublish
SO  - Cytokine. 2013 Jan;61(1):118-26. doi: 10.1016/j.cyto.2012.09.004. Epub 2012 Oct 
      9.