PMID- 23058661
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20131121
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 41
DP  - 2013 Mar 15
TI  - Au(III)-promoted magnetic molecularly imprinted polymer nanospheres for 
      electrochemical determination of streptomycin residues in food.
PG  - 551-6
LID - S0956-5663(12)00627-6 [pii]
LID - 10.1016/j.bios.2012.09.021 [doi]
AB  - Redox-active magnetic molecularly imprinted polymer (mMIP) nanospheres were first 
      synthesized and functionalized with streptomycin templates for highly efficient 
      electrochemical determination of streptomycin residues (STR) in food by coupling 
      with bioelectrocatalytic reaction of enzymes for signal amplification. The mMIP 
      nanospheres were synthesized by using Au(III)-promoted molecularly imprinted 
      polymerization with STR templates on magnetic beads. Based on extraction of 
      template molecules from the mMIP surface, the imprints toward STR templates were 
      formed. The assay was implemented with a competitive-type assay format. Upon 
      addition of streptomycin, the analyte competed with glucose oxidase-labeled 
      streptomycin (GOX-STR) for molecular imprints on the mMIP nanospheres. With the 
      increasing streptomycin in the sample, the conjugation amount of GOX-STR on the 
      mMIP nanospheres decreased, leading to the change in the bioelectrocatalytic 
      current relative to glucose system. Under optimal conditions, the catalytic 
      current was proportional to STR level in the sample, and exhibited a dynamic 
      range of 0.05-20 ng mL(-1) with a detection limit of 10 pg mL(-1) STR (at 3s(B)). 
      Intra- and inter-assay coefficients of variation were below 12%. The assayed 
      results for STR spiked samples including milk and honey with the mMIP-based 
      sensor were received a good accordance with the results obtained from the 
      referenced high-performance liquid chromatography (HPLC) method.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Liu, Bingqian
AU  - Liu B
AD  - Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education 
      and Fujian Province), Department of Chemistry and Chemical Engineering, Fuzhou 
      University, Fuzhou 350108, PR China.
FAU - Tang, Dianping
AU  - Tang D
FAU - Zhang, Bing
AU  - Zhang B
FAU - Que, Xiaohua
AU  - Que X
FAU - Yang, Huanghao
AU  - Yang H
FAU - Chen, Guonan
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120924
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 7440-57-5 (Gold)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Conductometry/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Food Analysis/*instrumentation
MH  - Food Contamination/*analysis
MH  - Gold/*chemistry
MH  - Microelectrodes
MH  - Molecular Imprinting/methods
MH  - Nanospheres/*chemistry
MH  - Nanotechnology/instrumentation
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Streptomycin/*analysis
EDAT- 2012/10/13 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/10/13 06:00
PHST- 2012/08/08 00:00 [received]
PHST- 2012/09/13 00:00 [revised]
PHST- 2012/09/14 00:00 [accepted]
PHST- 2012/10/13 06:00 [entrez]
PHST- 2012/10/13 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
AID - S0956-5663(12)00627-6 [pii]
AID - 10.1016/j.bios.2012.09.021 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Mar 15;41:551-6. doi: 10.1016/j.bios.2012.09.021. Epub 
      2012 Sep 24.