PMID- 23058982
OWN - NLM
STAT- MEDLINE
DCOM- 20130812
LR  - 20130218
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 94
IP  - 2
DP  - 2013 Apr
TI  - Positive inductive effect of IL-18 on virus-specific immune responses induced by 
      PRRSV-GP5 DNA vaccine in swine.
PG  - 346-53
LID - S0034-5288(12)00261-5 [pii]
LID - 10.1016/j.rvsc.2012.09.011 [doi]
AB  - Two DNA vaccines encoding the envelope protein GP5 of porcine reproductive and 
      respiratory syndrome virus (PRRSV) alone (pEGFP-GP5) and co-encoding GP5 and 
      swine interleukin-18 (IL-18) proteins (pEGFP-IL18-GP5), were constructed and 
      comparatively evaluated for their abilities to induce humoral and cellular 
      responses in piglets. Experimental results showed that among all vaccinated 
      groups, the live PRRSV vaccine elicited the highest titre of serum neutralizing 
      antibody and the strongest cell-mediated immune responses, which means that the 
      live PRRSV vaccine is still the first option for the vaccination against PRRSV. 
      Moreover, the piglets inoculated with pEGFP-IL18-GP5 developed significantly 
      higher IFN-gamma production response, significantly increased percentages of CD4(+) 
      and CD8(+) T-lymphocytes and significantly higher specific T-lymphocyte 
      proliferation response than the pEGFP-GP5 inoculated pigs (P<0.05). These results 
      demonstrated the positive inductive effect of IL-18 on the activation of cellular 
      immune responses in swines. Therefore, in order to develop a new type vaccine for 
      PRRS prevention and control, co-encoding of GP5 and IL-18 proteins may be a good 
      choice.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Zhang, Xiaodong
AU  - Zhang X
AD  - College of Animal Science and Veterinary Medicine, Key Laboratory of Zoonosis 
      Research, Ministry of Education, Institute of Zoonosis, Jilin University, 
      Changchun, Jilin 130062, China.
FAU - Li, Guojiang
AU  - Li G
FAU - Gao, Lei
AU  - Gao L
FAU - Mu, Lianzhi
AU  - Mu L
FAU - Zhang, Lichun
AU  - Zhang L
FAU - Cong, Yanlong
AU  - Cong Y
FAU - Ding, Zhuang
AU  - Ding Z
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20121008
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (DNA, Viral)
RN  - 0 (Interleukin-18)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - CD4-Positive T-Lymphocytes
MH  - CD8-Positive T-Lymphocytes
MH  - Cell Proliferation
MH  - DNA, Viral/immunology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - Interleukin-18/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Swine
MH  - Time Factors
MH  - Vaccines, DNA/immunology
MH  - Viral Envelope Proteins/*genetics/immunology
MH  - Viral Vaccines/*immunology
EDAT- 2012/10/13 06:00
MHDA- 2013/08/13 06:00
CRDT- 2012/10/13 06:00
PHST- 2012/02/23 00:00 [received]
PHST- 2012/07/27 00:00 [revised]
PHST- 2012/09/06 00:00 [accepted]
PHST- 2012/10/13 06:00 [entrez]
PHST- 2012/10/13 06:00 [pubmed]
PHST- 2013/08/13 06:00 [medline]
AID - S0034-5288(12)00261-5 [pii]
AID - 10.1016/j.rvsc.2012.09.011 [doi]
PST - ppublish
SO  - Res Vet Sci. 2013 Apr;94(2):346-53. doi: 10.1016/j.rvsc.2012.09.011. Epub 2012 
      Oct 8.