PMID- 23060870
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121015
LR  - 20211021
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 3
DP  - 2012
TI  - Archaeal dominated ammonia-oxidizing communities in Icelandic grassland soils are 
      moderately affected by long-term N fertilization and geothermal heating.
PG  - 352
LID - 10.3389/fmicb.2012.00352 [doi]
LID - 352
AB  - The contribution of ammonia-oxidizing bacteria and archaea (AOB and AOA, 
      respectively) to the net oxidation of ammonia varies greatly between terrestrial 
      environments. To better understand, predict and possibly manage terrestrial 
      nitrogen turnover, we need to develop a conceptual understanding of ammonia 
      oxidation as a function of environmental conditions including the ecophysiology 
      of associated organisms. We examined the discrete and combined effects of mineral 
      nitrogen deposition and geothermal heating on ammonia-oxidizing communities by 
      sampling soils from a long-term fertilization site along a temperature gradient 
      in Icelandic grasslands. Microarray, clone library and quantitative PCR analyses 
      of the ammonia monooxygenase subunit A (amoA) gene accompanied by 
      physico-chemical measurements of the soil properties were conducted. In contrast 
      to most other terrestrial environments, the ammonia-oxidizing communities 
      consisted almost exclusively of archaea. Their bacterial counterparts proved to 
      be undetectable by quantitative polymerase chain reaction suggesting AOB are only 
      of minor relevance for ammonia oxidation in these soils. Our results show that 
      fertilization and local, geothermal warming affected detectable ammonia-oxidizing 
      communities, but not soil chemistry: only a subset of the detected AOA phylotypes 
      was present in higher temperature soils and AOA abundance was increased in the 
      fertilized soils, while soil physio-chemical properties remained unchanged. 
      Differences in distribution and structure of AOA communities were best explained 
      by soil pH and clay content irrespective of temperature or fertilizer treatment 
      in these grassland soils, suggesting that these factors have a greater potential 
      for ecological niche-differentiation of AOA in soil than temperature and N 
      fertilization.
FAU - Daebeler, Anne
AU  - Daebeler A
AD  - Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW) 
      Wageningen, Netherlands ; Institute of Environmental Biology, University of 
      Utrecht Utrecht, Netherlands.
FAU - Abell, Guy C J
AU  - Abell GC
FAU - Bodelier, Paul L E
AU  - Bodelier PL
FAU - Bodrossy, Levente
AU  - Bodrossy L
FAU - Frampton, Dion M F
AU  - Frampton DM
FAU - Hefting, Mariet M
AU  - Hefting MM
FAU - Laanbroek, Hendrikus J
AU  - Laanbroek HJ
LA  - eng
PT  - Journal Article
DEP - 20121004
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC3463987
OTO - NOTNLM
OT  - ammonia-oxidizing archaea
OT  - ammonia-oxidizing bacteria
OT  - amoA
OT  - fertilization
OT  - grassland soil
OT  - niche formation
OT  - temperature
EDAT- 2012/10/13 06:00
MHDA- 2012/10/13 06:01
PMCR- 2012/10/04
CRDT- 2012/10/13 06:00
PHST- 2012/04/26 00:00 [received]
PHST- 2012/09/14 00:00 [accepted]
PHST- 2012/10/13 06:00 [entrez]
PHST- 2012/10/13 06:00 [pubmed]
PHST- 2012/10/13 06:01 [medline]
PHST- 2012/10/04 00:00 [pmc-release]
AID - 10.3389/fmicb.2012.00352 [doi]
PST - epublish
SO  - Front Microbiol. 2012 Oct 4;3:352. doi: 10.3389/fmicb.2012.00352. eCollection 
      2012.