PMID- 23062558
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20151119
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 41
DP  - 2013 Mar 15
TI  - Multiplexed sandwich immunoassays using flow-injection electrochemiluminescence 
      with designed substrate spatial-resolved technique for detection of tumor 
      markers.
PG  - 684-90
LID - S0956-5663(12)00650-1 [pii]
LID - 10.1016/j.bios.2012.09.044 [doi]
AB  - Convenient sensor array for simultaneous multi-analyte testing was increasingly 
      needed in clinical diagnosis. A novel electrochemiluminescence (ECL) immunosensor 
      array for the sequential detection of multiple tumor markers was developed by 
      site-selectively immobilizing multiple antigens on different electrodes. 
      Disposable indium tin oxide (ITO) glass array was employed as detection platform. 
      With a sandwich-type immunoassay format, the amount of carbon dots coated silica 
      (SiO(2)@C-dots) labeled antibodies increased with the increment of antigens in 
      the samples. The ECL signal from different immunosensors was collected in turn by 
      a photomultiplier (PMT) with the aid of a home-made potential transformer equiped 
      with a home-made multiplexed-switch. Using carcino embryonic antigen (CEA), 
      prostate specific antigen (PSA) and alpha-fetoprotein (alpha-AFP) as model analytes, the 
      proposed immunoassay exhibited excellent precision and sensitivity. For all three 
      analytes, the relative standard deviations (RSDs) for six times detection were 
      lower than 7.1% and the detection limits were in the range of 0.003-0.006 ng 
      mL(-1). The results for real sample analysis demonstrated that the newly 
      constructed immunosensor array provided a rapid, simple, simultaneous 
      multi-analyte immunoassay with high throughput, cost-effective and sufficiently 
      low detection limits for clinical applications. Importantly, the novel 
      individually addressable immunosensor array for multi-analyte immunoassay by 
      introducing the ECL readout mechanism with the aid of the home-made potential 
      transformer and multiplexed-switch could be a useful supplement to commercial 
      assay methods in clinical chemistry.
CI  - Copyright (c) 2012. Published by Elsevier B.V.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School 
      of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR 
      China.
FAU - Liu, Weiyan
AU  - Liu W
FAU - Ge, Shenguang
AU  - Ge S
FAU - Yan, Mei
AU  - Yan M
FAU - Wang, Shaowei
AU  - Wang S
FAU - Yu, Jinghua
AU  - Yu J
FAU - Li, Nianqiang
AU  - Li N
FAU - Song, Xianrang
AU  - Song X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120928
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Biomarkers, Tumor/*blood
MH  - Biosensing Techniques/instrumentation
MH  - Conductometry/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Flow Injection Analysis/*instrumentation
MH  - Humans
MH  - Immunoassay/*instrumentation
MH  - Luminescent Measurements/*instrumentation
MH  - Neoplasm Proteins/*blood
MH  - Neoplasms, Experimental/*blood/diagnosis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2012/10/16 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/07/22 00:00 [received]
PHST- 2012/09/09 00:00 [revised]
PHST- 2012/09/22 00:00 [accepted]
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
AID - S0956-5663(12)00650-1 [pii]
AID - 10.1016/j.bios.2012.09.044 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Mar 15;41:684-90. doi: 10.1016/j.bios.2012.09.044. Epub 
      2012 Sep 28.