PMID- 23062636
OWN - NLM
STAT- MEDLINE
DCOM- 20121204
LR  - 20191112
IS  - 1001-9294 (Print)
IS  - 1001-9294 (Linking)
VI  - 27
IP  - 3
DP  - 2012 Sep
TI  - Nucleotide-binding oligomerization domain-1 ligand induces inflammation and 
      attenuates glucose uptake in human adipocytes.
PG  - 147-52
AB  - OBJECTIVE: To investigate the effects of stimulant for nucleotide-binding 
      oligomerization domain 1 (NOD1) on secretion of proinflammatory chemokine / 
      cytokines and insulin-dependent glucose uptake in human differentiated 
      adipocytes. METHODS: Adipose tissues were obtained from patients undergoing 
      liposuction. Stromal vascular cells were extracted and differentiated into 
      adipocytes.A specific ligand for NOD1, was administered to human adipocytes in 
      culture. Nuclear factor-kappaB transcriptional activity and proinflammatory chemokine 
      / cytokines production were determined by reporter plasmid assay and 
      enzyme-linked immunosorbent assay, respectively.Insulin-stimulated glucose uptake 
      was measured by 2-deoxy-D-[(3)H] glucose uptake assay. Furthermore, chemokine / 
      cytokine secretion and glucose uptake in adipocytes transfected with small 
      interfering RNA (siRNA) targeting NOD1 upon stimulation of NOD1 ligand were 
      analyzed. RESULTS: Nuclear factor-kappaB transcriptional activity and monocyte 
      chemoattractant protein-1 (MCP-1), interleukin (IL)-6, and IL-8 secretion in 
      human adipocytes were markedly increased stimulated with NOD1 ligand (all 
      P<0.01).Insulin-induced glucose uptake was decreased upon the activation of NOD1 
      (P<0.05).NOD1 gene silencing by siRNA reduced NOD1 ligand-induced MCP-1,IL-6, and 
      IL-8 release and increased insulin-induced glucose uptake (all P<0.05). 
      CONCLUSION: NOD1 activation in adipocytes might be implicated in the onset of 
      insulin resistance.
FAU - Yi-Jun, Zhou
AU  - Yi-Jun Z
AD  - Department of Endocrinology and Metabolism, Fourth Affiliated Hospital of China 
      Medical University, Shenyang 110032, China. zhoudoctor@163.com
FAU - Ai, Li
AU  - Ai L
FAU - Yu-Ling, Song
AU  - Yu-Ling S
FAU - Yan, Li
AU  - Yan L
FAU - Hui, Yan
AU  - Hui Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med Sci J
JT  - Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih
JID - 9112559
RN  - 0 (Cytokines)
RN  - 0 (Ligands)
RN  - 0 (NF-kappa B)
RN  - 0 (NOD1 protein, human)
RN  - 0 (Nod1 Signaling Adaptor Protein)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Adult
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Glucose/*metabolism
MH  - Humans
MH  - Inflammation/*etiology
MH  - Insulin Resistance
MH  - Ligands
MH  - Middle Aged
MH  - NF-kappa B/physiology
MH  - Nod1 Signaling Adaptor Protein/*physiology
EDAT- 2012/10/16 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 9987 [pii]
AID - 10.1016/s1001-9294(14)60047-x [doi]
PST - ppublish
SO  - Chin Med Sci J. 2012 Sep;27(3):147-52. doi: 10.1016/s1001-9294(14)60047-x.