PMID- 23063069
OWN - NLM
STAT- MEDLINE
DCOM- 20130215
LR  - 20121231
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 126
DP  - 2013 Jan 15
TI  - Parental exposure to natural mixtures of POPs reduced embryo production and 
      altered gene transcription in zebrafish embryos.
PG  - 424-34
LID - S0166-445X(12)00247-0 [pii]
LID - 10.1016/j.aquatox.2012.08.019 [doi]
AB  - Determination of toxicity of complex mixtures has been proposed to be one of the 
      most important challenges for modern toxicology. In this study we performed 
      genome wide transcriptome profiling to assess potential toxicant induced changes 
      in gene regulation in zebrafish embryos following parental exposure to two 
      natural mixtures of persistent organic pollutants (POPs). The mixtures used were 
      extracted from burbot (Lota lota) liver originating from two lakes (Lake Mjosa 
      and Lake Losna) belonging to the same freshwater system in Norway. The dominating 
      groups of contaminants were polybrominated diphenyl ethers (PBDEs), 
      polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane metabolites 
      (DDTs). Because both mixtures used in the present study induced similar effects, 
      it is likely that the same toxicants are involved. The Mjosa mixture contains 
      high levels of PBDEs while this group of pollutants is low in the Losna mixture. 
      However, both mixtures contain substantial concentrations of PCB and DDT 
      suggesting these contaminants as the predominant contributors to the toxicity 
      observed. The observed effects included phenotypic traits, like embryo production 
      and survival, and gene transcription changes corresponding with disease and 
      biological functions such as cancer, reproductive system disease, cardiovascular 
      disease, lipid and protein metabolism, small molecule biochemistry and cell 
      cycle. The changes in gene transcription included genes regulated by HNF4A, 
      insulin, LH, FSH and NF-kappaB which are known to be central regulators of endocrine 
      signaling, metabolism, metabolic homeostasis, immune functions, cancer 
      development and reproduction. The results suggest that relative low 
      concentrations of the natural mixtures of POPs used in the present study might 
      pose a threat to wild freshwater fish living in the lakes from which the POPs 
      mixtures originated.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lyche, Jan L
AU  - Lyche JL
AD  - Dept. Food Safety and Infection Biology, Norwegian School of Veterinary Science, 
      Oslo, Norway. jan.l.lyche@nvh.no
FAU - Grzes, Irena M
AU  - Grzes IM
FAU - Karlsson, Camilla
AU  - Karlsson C
FAU - Nourizadeh-Lillabadi, Rasoul
AU  - Nourizadeh-Lillabadi R
FAU - Berg, Vidar
AU  - Berg V
FAU - Kristoffersen, Anja B
AU  - Kristoffersen AB
FAU - Skare, Janneche U
AU  - Skare JU
FAU - Alestrom, Peter
AU  - Alestrom P
FAU - Ropstad, Erik
AU  - Ropstad E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120920
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Embryo, Nonmammalian/drug effects
MH  - Gadiformes/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Liver/chemistry
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproduction/*drug effects
MH  - Time Factors
MH  - Water Pollutants, Chemical/*toxicity
MH  - *Zebrafish
EDAT- 2012/10/16 06:00
MHDA- 2013/02/16 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/06/15 00:00 [received]
PHST- 2012/08/22 00:00 [revised]
PHST- 2012/08/25 00:00 [accepted]
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2013/02/16 06:00 [medline]
AID - S0166-445X(12)00247-0 [pii]
AID - 10.1016/j.aquatox.2012.08.019 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2013 Jan 15;126:424-34. doi: 10.1016/j.aquatox.2012.08.019. Epub 
      2012 Sep 20.