PMID- 23065281
OWN - NLM
STAT- MEDLINE
DCOM- 20130617
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 40
IP  - 2
DP  - 2013 Feb
TI  - Involvement of ZEB1 and E-cadherin in the invasion of lung squamous cell 
      carcinoma.
PG  - 949-56
LID - 10.1007/s11033-012-2136-4 [doi]
AB  - This study intended to investigate the expression of the ZEB1 and E-cadherin 
      proteins in lung squamous cell carcinoma (LSCC) tissues and to examine the 
      clinicopathological correlation between protein levels and LSCC. RT-PCR and 
      Western blot were used to examine the expression of ZEB1 and E-cadherin mRNAs and 
      proteins in LSCC tissues as well as in adjacent normal tissues, and then analyze 
      the relationship between the clinicopathological characteristics and the 
      expression changes of ZEB1 and E-cadherin mRNAs in LSCC. In addition, RNAi was 
      used to knockdown the expression of the ZEB1 gene in Human HCC827 cells; 
      subsequently, changes in the invasive ability of the resultant cells were 
      studied. The positive rates of ZEB1 and E-cadherin mRNAs in LSCC tissues were 
      69.2 and 38.5 %, respectively. They differed significantly from the corresponding 
      positive rates in the adjacent normal lung tissues (15.4 and 80.8 %, p < 0.05). 
      There was a negative correlation between the protein levels of ZEB1 and 
      E-cadherin in LSCC tissues (r = -0.714, p < 0.001); in addition, it was found 
      that ZEB1 protein expression in LSCC tissues was significantly higher than that 
      in the neighboring normal lung tissues (p < 0.05), and its expression was also 
      significantly higher in patients with lymph node metastases and distant 
      metastases compared to those patients without metastatic disease (p < 0.05). On 
      the contrary, E-cadherin expression was significantly lower in LSCC tissues than 
      that in the neighboring normal tissue (p < 0.05). It was lower in patients with 
      lymph node metastasis and distant metastasis compared to patients without 
      metastatic disease (p < 0.05). However, the expression of ZEB1 and E-cadherin was 
      independent of gender, age, tumor size, or tumor differentiation level 
      (p > 0.05). Transfection of ZEB1 siRNA into HCC827 cells significantly reduced 
      the ZEB1 protein level (p < 0.01) and significantly elevated E-cadherin levels 
      (p < 0.01). Moreover, significantly less ZEB1 siRNA-transfected cells migrated 
      through Transwell chambers in the LSCC tissue than that in the control groups 
      (untransfected or transfected with control siRNA, p < 0.01). The expression of 
      the ZEB1 gene in LSCC tissues is downregulated with the expression of E-cadherin. 
      On the other hand, the expression of siRNA against ZEB1 promotes E-cadherin 
      expression and suppresses the invasive ability conferred by E-cadherin. In 
      conclusion, our data suggested that overexpression of the ZEB1 gene is possibly 
      associated with the occurrence, development, invasion of LSCC.
FAU - Zhang, Jiaxing
AU  - Zhang J
AD  - Department of Interventional Radiology, Shanghai 10th People's Hospital, Tongji 
      University, No. 301, Yanchang Road, Shanghai, 200072, China. zhangjuntj@yeah.net
FAU - Lu, Chenhui
AU  - Lu C
FAU - Zhang, Jun
AU  - Zhang J
FAU - Kang, Jiuhong
AU  - Kang J
FAU - Cao, Chuanwu
AU  - Cao C
FAU - Li, Maoquan
AU  - Li M
LA  - eng
PT  - Journal Article
DEP - 20121014
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Transcription Factors)
RN  - 0 (ZEB1 protein, human)
RN  - 0 (Zinc Finger E-box-Binding Homeobox 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD
MH  - Cadherins/genetics/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Female
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - RNA, Small Interfering/genetics
MH  - Transcription Factors/genetics/*metabolism
MH  - Zinc Finger E-box-Binding Homeobox 1
EDAT- 2012/10/16 06:00
MHDA- 2013/06/19 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/07/11 00:00 [received]
PHST- 2012/10/03 00:00 [accepted]
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - 10.1007/s11033-012-2136-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2013 Feb;40(2):949-56. doi: 10.1007/s11033-012-2136-4. Epub 2012 
      Oct 14.