PMID- 23065402
OWN - NLM
STAT- MEDLINE
DCOM- 20130516
LR  - 20121205
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 168
IP  - 7
DP  - 2012 Dec
TI  - Metabolic flux and nodes control analysis of brewer's yeasts under different 
      fermentation temperature during beer brewing.
PG  - 1938-52
LID - 10.1007/s12010-012-9909-z [doi]
AB  - The aim of this work was to further investigate the glycolysis performance of 
      lager and ale brewer's yeasts under different fermentation temperature using a 
      combined analysis of metabolic flux, glycolytic enzyme activities, and flux 
      control. The results indicated that the fluxes through glycolytic pathway 
      decreased with the change of the fermentation temperature from 15  degrees C to 10  degrees C, 
      which resulted in the prolonged fermentation times. The maximum activities (V 
      (max)) of hexokinase (HK), phosphofructokinase (PFK), and pyruvate kinase (PK) at 
      key nodes of glycolytic pathway decreased with decreasing fermentation 
      temperature, which was estimated to have different control extent (22-84 %) on 
      the glycolytic fluxes in exponential or flocculent phase. Moreover, the decrease 
      of V (max) of PFK or PK displayed the crucial role in down-regulation of flux in 
      flocculent phase. In addition, the metabolic state of ale strain was more 
      sensitive to the variation of temperature than that of lager strain. The results 
      of the metabolic flux and nodes control analysis in brewer's yeasts under 
      different fermentation temperature may provide an alternative approach to 
      regulate glycolytic flux by changing V (max) and improve the production 
      efficiency and beer quality.
FAU - Yu, Zhimin
AU  - Yu Z
AD  - College of Light Industry and Food Sciences, South China University of 
      Technology, Guangzhou 510640, People's Republic of China.
FAU - Zhao, Haifeng
AU  - Zhao H
FAU - Zhao, Mouming
AU  - Zhao M
FAU - Lei, Hongjie
AU  - Lei H
FAU - Li, Huiping
AU  - Li H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121012
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
SB  - IM
MH  - Beer/*microbiology
MH  - *Fermentation
MH  - Glycolysis
MH  - Phosphotransferases (Alcohol Group Acceptor)/metabolism
MH  - Saccharomyces cerevisiae/enzymology/*metabolism
MH  - Species Specificity
MH  - *Temperature
EDAT- 2012/10/16 06:00
MHDA- 2013/05/17 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/02/10 00:00 [received]
PHST- 2012/10/03 00:00 [accepted]
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2013/05/17 06:00 [medline]
AID - 10.1007/s12010-012-9909-z [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2012 Dec;168(7):1938-52. doi: 10.1007/s12010-012-9909-z. 
      Epub 2012 Oct 12.