PMID- 23065866
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20151119
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 101
IP  - 6
DP  - 2013 Jun
TI  - In vitro cytotoxicity and in vivo osseointergration properties of 
      compression-molded HDPE-HA-Al2O3 hybrid biocomposites.
PG  - 1539-49
LID - 10.1002/jbm.a.34452 [doi]
AB  - The aim of this study was to investigate the in vivo biocompatibility in terms of 
      healing of long segmental bone defect in rabbit model as well as in vitro 
      cytotoxicity of eluates of compression-molded High density polyethylene 
      (HDPE)-hydroxyapatite (HA)-aluminum oxide (Al2O3) composite-based implant 
      material. Based on the physical property in terms of modulus and strength 
      properties, as reported in our recent publication, HDPE-40 wt % HA and HDPE-20 wt 
      % HA-20 wt % Al2O3 hybrid composites were used for biocompatibility assessment. 
      Osteoblasts cells were cultured in conditioned media, which contains varying 
      amount of composite eluate (0.01, 0.1, and 1.0 wt %). In vitro, the eluates did 
      not exhibit any significant negative impact on proliferation, mineralization or 
      on morphology of human osteoblast cells. In vivo, the histological assessment 
      revealed neobone formation at the bone/implant interface, characterized by the 
      presence of osteoid and osteoblasts. The observation of osteoclastic activity 
      indicates the process of bone remodeling. No inflammation to any noticeable 
      extent was observed at the implantation site. Overall, the combination of in 
      vitro and in vivo results are suggestive of potential biomedical application of 
      compression-molded HDPE- 20 wt % HA- 20 wt % Al2O3 composites to heal long 
      segmental bone defects without causing any toxicity of bone cells.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Tripathi, Garima
AU  - Tripathi G
AD  - Department of Materials Science and Engineering, Laboratory for Biomaterials, 
      Indian Institute of Technology, Kanpur 208016, Uttra Pradesh, India.
FAU - Gough, Julie E
AU  - Gough JE
FAU - Dinda, Amit
AU  - Dinda A
FAU - Basu, Bikramjit
AU  - Basu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121015
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Anthraquinones)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Oxazines)
RN  - 0 (Xanthenes)
RN  - 1FN9YD6968 (resazurin)
RN  - 60MEW57T9G (alizarin)
RN  - 9002-88-4 (Polyethylene)
RN  - 91D9GV0Z28 (Durapatite)
RN  - LMI26O6933 (Aluminum Oxide)
SB  - IM
MH  - Aluminum Oxide/*pharmacology
MH  - Animals
MH  - Anthraquinones/metabolism
MH  - Biocompatible Materials/*pharmacology
MH  - Bone and Bones/drug effects/ultrastructure
MH  - Calcification, Physiologic/drug effects
MH  - Cell Count
MH  - Cell Death/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - *Compressive Strength
MH  - Culture Media, Conditioned/pharmacology
MH  - Durapatite/*pharmacology
MH  - Humans
MH  - Implants, Experimental
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - Osseointegration/*drug effects
MH  - Osteoblasts/*cytology/drug effects/metabolism
MH  - Oxazines/metabolism
MH  - Polyethylene/*pharmacology
MH  - Rabbits
MH  - X-Ray Diffraction
MH  - Xanthenes/metabolism
EDAT- 2012/10/16 06:00
MHDA- 2013/10/23 06:00
CRDT- 2012/10/16 06:00
PHST- 2012/04/19 00:00 [received]
PHST- 2012/07/18 00:00 [revised]
PHST- 2012/08/28 00:00 [accepted]
PHST- 2012/10/16 06:00 [entrez]
PHST- 2012/10/16 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - 10.1002/jbm.a.34452 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2013 Jun;101(6):1539-49. doi: 10.1002/jbm.a.34452. Epub 
      2012 Oct 15.