PMID- 23067401
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121107
LR  - 20211021
IS  - 1757-2215 (Electronic)
IS  - 1757-2215 (Linking)
VI  - 5
IP  - 1
DP  - 2012 Oct 15
TI  - The effects of DNA methylation and epigenetic factors on the expression of CD133 
      in ovarian cancers.
PG  - 28
LID - 10.1186/1757-2215-5-28 [doi]
AB  - BACKGROUND: To identifying the effects of DNA methylation and epigenetic factors 
      on the expression of CD133, a cancer stem cell marker, in gynecologic cancer cell 
      lines. METHODS: Ovarian cancer cell lines (OVCAR-8 and IGROV-1) and an 
      endometrial cancer cell line (Ishikawa) were treated with 5-aza-2;-deoxycytidine 
      (DAC) or Trichostatin A (TSA). Expression of CD133 was evaluated by quantitative 
      real-time PCR, methylation-specific PCR (MSP), reverse transcription-PCR, western 
      blot, and FACS analysis. All results are representative of three independent 
      experiments. RESULTS: CD133 mRNA expression varied among the different cell 
      lines; the weakest expression was observed in OVCAR-8 cells, while it was 
      strongly expressed in Ishikawa cells. The degree of methylation of the CD133 P2 
      promoter was 61% in OVCAR-8 cells, 53% in IGROV-1 cells, and 43% in Ishikawa 
      cells. CD133 expression was increased at both the mRNA and protein level after 
      DAC treatment. On the contrary, CD133 mRNA expression decreased after TSA 
      treatment decreased in all cell lines except OVCAR-8. In addition, MSP of the 
      CD133 P2 promoter revealed that methylation was reduced after treatment with 
      either DAC or TSA. CONCLUSIONS: The expression of the CD133 antigen in primary 
      ovarian and endometrial cancer cell lines is regulated by epigenetics, as 
      indicated by its increased expression following DAC treatment and irregular 
      expression pattern followed by TSA treatment. In addition, the expression of 
      CD133 was negatively correlated with the degree of methylation of the CD133 P2 
      promoter.
FAU - Min, Kyung-Jin
AU  - Min KJ
AD  - Department of Obstetrics and Gynecology, Guro Hospital, College of Medicine, 
      Korea University, Seoul, Korea. jklee38@gmail.com.
FAU - So, Kyeong A
AU  - So KA
FAU - Ouh, Yung-Taek
AU  - Ouh YT
FAU - Hong, Jin-Hwa
AU  - Hong JH
FAU - Lee, Jae-Kwan
AU  - Lee JK
LA  - eng
PT  - Journal Article
DEP - 20121015
PL  - England
TA  - J Ovarian Res
JT  - Journal of ovarian research
JID - 101474849
PMC - PMC3489563
EDAT- 2012/10/17 06:00
MHDA- 2012/10/17 06:01
PMCR- 2012/10/15
CRDT- 2012/10/17 06:00
PHST- 2012/09/07 00:00 [received]
PHST- 2012/10/03 00:00 [accepted]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2012/10/17 06:01 [medline]
PHST- 2012/10/15 00:00 [pmc-release]
AID - 1757-2215-5-28 [pii]
AID - 10.1186/1757-2215-5-28 [doi]
PST - epublish
SO  - J Ovarian Res. 2012 Oct 15;5(1):28. doi: 10.1186/1757-2215-5-28.