PMID- 23070471
OWN - NLM
STAT- MEDLINE
DCOM- 20130529
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 38
IP  - 1
DP  - 2013 Jan
TI  - Treatment in vitro of retinal cells with IL-4 increases the survival of retinal 
      ganglion cells: the involvement of BDNF.
PG  - 162-73
LID - 10.1007/s11064-012-0904-0 [doi]
AB  - Interleukin 4 (IL-4) is a pleiotropic cytokine involved in many functions during 
      the development as well as in adult life. Previous work from our group 
      demonstrated, in vitro, that this interleukin is able to prevent rat retinal 
      ganglion cells death after axotomy. The aim of the present study was to 
      investigate the signaling pathways involved in this trophic effect, particularly 
      the cAMP pathway and also to demonstrate the expression of IL-4 in retinas at 
      different stages of post natal development. Our results show that the trophic 
      effect of IL-4 on rat retinal ganglion cells is dependent on the activation of 
      Janus Kinase 3, Protein Kinase A, c-Jun N-terminal Kinase and Tropomyosin related 
      Kinase receptors, on the increase in intracellular calcium levels, on polypeptide 
      release and on the endogenous Brain Derived Neurotrophic Factor (BDNF). We also 
      observed that treatment with IL-4 enhances c-AMP response element binding and 
      Mitogen Activated Protein Kinase phosphorylation and increases the expression of 
      BDNF. Concerning the IL-4 expression our data show an increase in IL-4 levels 
      during post natal development. Taken together our results demonstrate that the 
      trophic effect of IL-4 on retinal ganglion cells of newborn rats is mediated by 
      cAMP pathway and BDNF release.
FAU - de Araujo-Martins, Leandro
AU  - de Araujo-Martins L
AD  - Programa de Neurociencias, Departamento de Neurobiologia, Instituto de Biologia, 
      Universidade Federal Fluminense, Outeiro de Sao Joao Batista s/n, Niteroi, Rio de 
      Janeiro CEP 24020-140, Brazil.
FAU - de Oliveira, Raphael Monteiro
AU  - de Oliveira RM
FAU - dos Santos, Gabriela Velozo Gomes
AU  - dos Santos GV
FAU - dos Santos, Renata Claudia Celestino
AU  - dos Santos RC
FAU - dos Santos, Aline Araujo
AU  - dos Santos AA
FAU - Giestal de Araujo, Elizabeth
AU  - Giestal de Araujo E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121016
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 207137-56-2 (Interleukin-4)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Cell Count
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cyclic AMP/physiology
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Enzyme Activation/drug effects/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Mitogen-Activated Protein Kinases/physiology
MH  - Phosphorylation/physiology
MH  - Protein Kinase C/antagonists & inhibitors/physiology
MH  - Rats
MH  - Retina/cytology/*drug effects/transplantation
MH  - Retinal Ganglion Cells/*drug effects
MH  - Signal Transduction/drug effects
EDAT- 2012/10/17 06:00
MHDA- 2013/05/31 06:00
CRDT- 2012/10/17 06:00
PHST- 2012/05/02 00:00 [received]
PHST- 2012/10/04 00:00 [accepted]
PHST- 2012/07/24 00:00 [revised]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2013/05/31 06:00 [medline]
AID - 10.1007/s11064-012-0904-0 [doi]
PST - ppublish
SO  - Neurochem Res. 2013 Jan;38(1):162-73. doi: 10.1007/s11064-012-0904-0. Epub 2012 
      Oct 16.