PMID- 23070877
OWN - NLM
STAT- MEDLINE
DCOM- 20130610
LR  - 20130102
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 76
IP  - 1
DP  - 2013 Jan
TI  - Light regulates the expression of the BDNF/TrkB system in the adult zebrafish 
      retina.
PG  - 42-9
LID - 10.1002/jemt.22133 [doi]
AB  - The retina of the adult zebrafish express brain-derived neurotrophic factor 
      (BDNF) and its signaling receptor TrkB. This functional system is involved in the 
      biology of the vertebrate retina and its expression is regulated by light. This 
      study was designed to investigate the effects of cyclic (12 h light/12 h 
      darkness) or continuous (24 h) exposure during 10 days to white light, white-blue 
      light, and blue light, as well as of darkness, on the expression of BDNF and TrkB 
      in the retina. BDNF and TrkB were assessed in the retina of adult zebrafish using 
      quantitative real-time polymerase chain reaction and immunohistochemistry. 
      Exposure to white, white-blue, and blue light causes a decrease of BDNF mRNA and 
      of BDNF immunostaining, independently of the pattern of light exposition. 
      Conversely, in the same experimental conditions, the expression of TrkB mRNA was 
      upregulated and TrkB immunostaining increased. Exposition to darkness diminished 
      BDNF and TrkB mRNAs, and abolished the immunostaining for BDNF but not modified 
      that for TrkB. These results demonstrate the regulation of BDNF and TrkB by light 
      in the retina of adult zebrafish and might contribute to explain some aspects of 
      the complex pathophysiology of light-induced retinopathies.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Sanchez-Ramos, C
AU  - Sanchez-Ramos C
AD  - Departamento de Optica II (Optometria y Vision), Universidad Complutense de 
      Madrid, Madrid, Spain.
FAU - Bonnin-Arias, C
AU  - Bonnin-Arias C
FAU - Guerrera, M C
AU  - Guerrera MC
FAU - Calavia, M G
AU  - Calavia MG
FAU - Chamorro, E
AU  - Chamorro E
FAU - Montalbano, G
AU  - Montalbano G
FAU - Lopez-Velasco, S
AU  - Lopez-Velasco S
FAU - Lopez-Muniz, A
AU  - Lopez-Muniz A
FAU - Germana, A
AU  - Germana A
FAU - Vega, J A
AU  - Vega JA
LA  - eng
PT  - Journal Article
DEP - 20121016
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Zebrafish Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Darkness
MH  - Female
MH  - Gene Expression Regulation, Developmental/radiation effects
MH  - Light
MH  - Male
MH  - Receptor, trkB/*genetics/metabolism
MH  - Retina/growth & development/metabolism/*radiation effects
MH  - Zebrafish/*genetics/growth & development/metabolism
MH  - Zebrafish Proteins/*genetics/metabolism
EDAT- 2012/10/17 06:00
MHDA- 2013/06/12 06:00
CRDT- 2012/10/17 06:00
PHST- 2012/07/16 00:00 [received]
PHST- 2012/09/12 00:00 [accepted]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - 10.1002/jemt.22133 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2013 Jan;76(1):42-9. doi: 10.1002/jemt.22133. Epub 2012 Oct 16.