PMID- 23071706
OWN - NLM
STAT- MEDLINE
DCOM- 20130501
LR  - 20230313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Identification and function of myeloid differentiation factor 88 (MyD88) in 
      Litopenaeus vannamei.
PG  - e47038
LID - 10.1371/journal.pone.0047038 [doi]
LID - e47038
AB  - Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling 
      protein in Toll-like receptor/interleukin-1 receptor-induced activation of 
      nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and 
      LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp 
      in length and contains an open reading frame (ORF) of 1,428 bp, whereas the 
      LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants 
      encode proteins with death and Toll/interleukin-1 receptor domains and share 91% 
      sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly 
      expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s 
      expression was induced in hemocytes after challenge with lipopolysaccharide, 
      CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot 
      syndrome virus, but not by poly IratioC. Overexpression of LvMyD88 and LvMyD88-1 in 
      Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and 
      wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a 
      role in antibacterial and antiviral response in L. vannamei. To our knowledge, 
      this is the first report on MyD88 in shrimp and a variant of MyD88 gene in 
      invertebrates.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of 
      China.
FAU - Li, Chao-Zheng
AU  - Li CZ
FAU - Yan, Hui
AU  - Yan H
FAU - Qiu, Wei
AU  - Qiu W
FAU - Chen, Yong-Gui
AU  - Chen YG
FAU - Wang, Pei-Hui
AU  - Wang PH
FAU - Weng, Shao-Ping
AU  - Weng SP
FAU - He, Jian-Guo
AU  - He JG
LA  - eng
SI  - GENBANK/JX073566
SI  - GENBANK/JX073567
SI  - GENBANK/JX073568
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121012
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antimicrobial Cationic Peptides/genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Drosophila/cytology/genetics
MH  - Gene Expression Regulation
MH  - Hemocytes/drug effects/physiology/virology
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - Myeloid Differentiation Factor 88/*genetics/*metabolism
MH  - Penaeidae/*genetics/*immunology/metabolism/microbiology/virology
MH  - Phylogeny
MH  - Promoter Regions, Genetic
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Staphylococcus aureus/pathogenicity
MH  - Vibrio parahaemolyticus/pathogenicity
MH  - White spot syndrome virus 1/pathogenicity
PMC - PMC3470552
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/10/17 06:00
MHDA- 2013/05/02 06:00
PMCR- 2012/10/12
CRDT- 2012/10/17 06:00
PHST- 2012/05/26 00:00 [received]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2013/05/02 06:00 [medline]
PHST- 2012/10/12 00:00 [pmc-release]
AID - PONE-D-12-15373 [pii]
AID - 10.1371/journal.pone.0047038 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e47038. doi: 10.1371/journal.pone.0047038. Epub 2012 Oct 12.