PMID- 23071783
OWN - NLM
STAT- MEDLINE
DCOM- 20130404
LR  - 20240313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Influence of androgens on circulating adiponectin in male and female rodents.
PG  - e47315
LID - 10.1371/journal.pone.0047315 [doi]
LID - e47315
AB  - Several endocrine factors, including sex-steroid hormones are known to influence 
      adiponectin secretion. Our purpose was to evaluate the influence of testosterone 
      and of the synthetic non-aromatizable/non-5alpha reducible androgen 
      17beta-hydroxyestra-4,9,11-trien-3-one (trenbolone) on circulating adiponectin and 
      adiponectin protein expression within visceral fat. Young male and female F344 
      rats underwent sham surgery (SHAM), gonadectomy (GX), or GX plus supraphysiologic 
      testosterone-enanthate (TE) administration. Total circulating adiponectin was 39% 
      higher in intact SHAM females than SHAM males (p<0.05). GX increased total 
      adiponectin by 29-34% in both sexes (p<0.05), while TE reduced adiponectin to 
      concentrations that were 46-53% below respective SHAMs (p</=0.001) and ablated the 
      difference in adiponectin between sexes. No differences in high molecular weight 
      (HMW) adiponectin were observed between sexes or treatments. Adiponectin 
      concentrations were highly and negatively associated with serum testosterone 
      (males: r = -0.746 and females: r = -0.742, p</=0.001); however, no association was 
      present between adiponectin and estradiol. In separate experiments, 
      trenbolone-enanthate (TREN) prevented the GX-induced increase in serum 
      adiponectin (p</=0.001) in young animals, with Low-dose TREN restoring adiponectin 
      to the level of SHAMs and higher doses of TREN reducing adiponectin to below SHAM 
      concentrations (p</=0.001). Similarly, TREN reduced adiponectin protein expression 
      within visceral fat (p<0.05). In adult GX males, Low-dose TREN also reduced total 
      adiponectin and visceral fat mass to a similar magnitude as TE, while increasing 
      serum HMW adiponectin above SHAM and GX animals (p<0.05). Serum adiponectin was 
      positively associated with visceral fat mass in young (r = 0.596, p</=0.001) and 
      adult animals (r = 0.657, p</=0.001). Our results indicate that androgens reduce 
      circulating total adiponectin concentrations in a dose-dependent manner, while 
      maintaining HMW adiponectin. This change is directionally similar to the 
      androgen-induced lipolytic effects on visceral adiposity and equal in magnitude 
      between TE and TREN, suggesting that neither the aromatization nor the 5alpha 
      reduction of androgens is required for this effect.
FAU - Yarrow, Joshua F
AU  - Yarrow JF
AD  - Malcom Randall Veterans Affairs Medical Center, Gainesville, FL, USA. 
      jfyarrow@ufl.edu
FAU - Beggs, Luke A
AU  - Beggs LA
FAU - Conover, Christine F
AU  - Conover CF
FAU - McCoy, Sean C
AU  - McCoy SC
FAU - Beck, Darren T
AU  - Beck DT
FAU - Borst, Stephen E
AU  - Borst SE
LA  - eng
GR  - P30 AG028740/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121010
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Adiponectin)
RN  - 0 (Placebos)
RN  - 3XMK78S47O (Testosterone)
RN  - RUD5Y4SV0S (Trenbolone Acetate)
SB  - IM
MH  - Adiponectin/*blood
MH  - Animals
MH  - Female
MH  - Male
MH  - Placebos
MH  - Rats
MH  - Rats, Inbred F344
MH  - Sex Factors
MH  - Testosterone/*pharmacology
MH  - Trenbolone Acetate/*pharmacology
PMC - PMC3468547
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/10/17 06:00
MHDA- 2013/04/05 06:00
PMCR- 2012/10/10
CRDT- 2012/10/17 06:00
PHST- 2012/02/02 00:00 [received]
PHST- 2012/09/14 00:00 [accepted]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2013/04/05 06:00 [medline]
PHST- 2012/10/10 00:00 [pmc-release]
AID - PONE-D-12-03781 [pii]
AID - 10.1371/journal.pone.0047315 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e47315. doi: 10.1371/journal.pone.0047315. Epub 2012 Oct 10.