PMID- 23073806
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20220726
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 32
IP  - 5
DP  - 2012 Oct
TI  - 1,2,3,4,6-penta-O-galloyl-beta-D-glucose protects PC12 Cells from MPP(+)-mediated 
      cell death by inducing heme oxygenase-1 in an ERK- and Akt-dependent manner.
PG  - 737-745
LID - 10.1007/s11596-012-1027-1 [doi]
AB  - This study examined the ability of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (beta-PGG) 
      to induce the expression of heme oxygenase-1 (HO-1) in the PC12 cells and its 
      regulation in the PC12 cells. One week before treatment with the drug, nerve 
      growth factor (NGF) was added to the cultures at a final concentration of 50 
      ng/mL to induce neuronal differentiation. After drug treatment, HO-1 gene 
      transcription was analyzed by reverse transcription polymerase chain reaction 
      (RT-PCR). Expression of HO-1 and NF-E2-related factor2 (Nrf2) and activation of 
      extracellular signal-regulated kinase (ERK) and Akt were detected by Western 
      blotting. The viability of the PC12 cells treated with different medicines was 
      examined by MTT assay. The oxidative stress in the PC12 cells was evaluated 
      qualitatively and quantitatively by DCFH-DA. The results showed that beta-PGG 
      up-regulated HO-1 expression and this increased expression provided 
      neuroprotection against MPP(+)-induced oxidative injury. Moreover, beta-PGG induced 
      Nrf2 nuclear translocation, which was found to be upstream of beta-PGG-induced HO-1 
      expression, and the activation of ERK and Akt, a pathway that is involved in 
      beta-PGG-induced Nrf2 nuclear translocation, HO-1 expression and neuroprotection. In 
      conclusion, beta-PGG up-regulates HO-1 expression by stimulating Nrf2 nuclear 
      translocation in an ERK- and Akt-dependent manner, and HO-1 expression by beta-PGG 
      may provide the PC12 cells with an acquired antioxidant defense capacity to 
      survive the oxidative stress.
FAU - Chen, Hong
AU  - Chen H
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Li, Hongge
AU  - Li H
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Cao, Fei
AU  - Cao F
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Zhen, Lan
AU  - Zhen L
AD  - Department of Obstetrics and Gynecology, Maternal and Child Health Hospital of 
      Fujian Province, Fuzhou, 350001, China.
FAU - Bai, Jing
AU  - Bai J
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Yuan, Shijin
AU  - Yuan S
AD  - Division of Histology and Embryology, Department of Anatomy, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, 430030, China.
FAU - Mei, Yuanwu
AU  - Mei Y
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China. lnlhckao@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121018
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 
      (1,3-bis(4-hydroxyphenyl)-4-methyl-5-(4-(2-piperidinylethoxy)phenol)-1H-pyrazole)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Piperidines)
RN  - 0 (Pyrazoles)
RN  - 3UI3K8W93I (pentagalloylglucose)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Cell Death/*drug effects/genetics
MH  - Cell Line, Tumor
MH  - Heme Oxygenase-1/*genetics
MH  - Hydrolyzable Tannins/*pharmacology
MH  - MAP Kinase Signaling System/*drug effects/genetics
MH  - PC12 Cells
MH  - Piperidines/*adverse effects
MH  - Proto-Oncogene Proteins c-akt/*genetics
MH  - Pyrazoles/*adverse effects
MH  - Rats
EDAT- 2012/10/18 06:00
MHDA- 2014/09/10 06:00
CRDT- 2012/10/18 06:00
PHST- 2012/02/21 00:00 [received]
PHST- 2012/10/18 06:00 [entrez]
PHST- 2012/10/18 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1007/s11596-012-1027-1 [pii]
AID - 10.1007/s11596-012-1027-1 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):737-745. doi: 
      10.1007/s11596-012-1027-1. Epub 2012 Oct 18.