PMID- 23076128
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20231213
IS  - 1347-8648 (Electronic)
IS  - 1347-8613 (Linking)
VI  - 120
IP  - 3
DP  - 2012
TI  - Imipramine induces brain-derived neurotrophic factor mRNA expression in cultured 
      astrocytes.
PG  - 176-86
AB  - Depression is one of the most prevalent and livelihood-threatening forms of 
      mental illnesses and the neural circuitry underlying depression remains 
      incompletely understood. Recent studies suggest that the neuronal plasticity 
      involved with brain-derived neurotrophic factor (BDNF) plays an important role in 
      the recovery from depression. Some antidepressants are reported to induce BDNF 
      expression in vivo; however, the mechanisms have been considered solely in 
      neurons and not fully elucidated. In the present study, we evaluated the effects 
      of imipramine, a classic tricyclic antidepressant drug, on BDNF expression in 
      cultured rat brain astrocytes. Imipramine dose-dependently increased BDNF mRNA 
      expression in astrocytes. The imipramine-induced BDNF increase was suppressed 
      with inhibitors for protein kinase A (PKA) or MEK/ERK. Moreover, imipramine 
      exposure activated transcription factor cAMP response element binding protein 
      (CREB) in a dose-dependent manner. These results suggested that imipramine 
      induced BDNF expression through CREB activation via PKA and/or ERK pathways. 
      Imipramine treatment in depression might exert antidepressant action through BDNF 
      production from astrocytes, and glial BDNF expression might be a target of 
      developing novel antidepressants.
FAU - Takano, Katsura
AU  - Takano K
AD  - Laboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture 
      University, Izumisano, Osaka, Japan. takano@vet.osakafu-u.ac.jp
FAU - Yamasaki, Hiroshi
AU  - Yamasaki H
FAU - Kawabe, Kenji
AU  - Kawabe K
FAU - Moriyama, Mitsuaki
AU  - Moriyama M
FAU - Nakamura, Yoichi
AU  - Nakamura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121016
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Adrenergic Uptake Inhibitors)
RN  - 0 (Antidepressive Agents, Tricyclic)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Creb1 protein, rat)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - OGG85SX4E4 (Imipramine)
SB  - IM
MH  - Adrenergic Uptake Inhibitors/*pharmacology
MH  - Animals
MH  - Antidepressive Agents, Tricyclic/*pharmacology
MH  - Astrocytes/cytology/*drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cyclic AMP Response Element-Binding Protein/agonists/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors/metabolism
MH  - Down-Regulation/drug effects
MH  - Embryo, Mammalian
MH  - Hippocampus/cytology/*drug effects/metabolism
MH  - Imipramine/*pharmacology
MH  - MAP Kinase Signaling System/drug effects
MH  - Nerve Tissue Proteins/agonists/antagonists & inhibitors/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Protein Processing, Post-Translational/drug effects
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Up-Regulation/*drug effects
EDAT- 2012/10/19 06:00
MHDA- 2013/04/23 06:00
CRDT- 2012/10/19 06:00
PHST- 2012/10/19 06:00 [entrez]
PHST- 2012/10/19 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
AID - DN/JST.JSTAGE/jphs/12039FP [pii]
AID - 10.1254/jphs.12039fp [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2012;120(3):176-86. doi: 10.1254/jphs.12039fp. Epub 2012 Oct 16.