PMID- 23076184
OWN - NLM
STAT- MEDLINE
DCOM- 20130725
LR  - 20121018
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 32
IP  - 10
DP  - 2012 Oct
TI  - [Optimization of pre-coated multi-probe fluorescence in situ hybridization for 
      cytogenetic detection of acute leukemia].
PG  - 1457-60
AB  - OBJECTIVE: To optimize pre-coated multiple-probe fluorescence in situ 
      hybridization (FISH) to improve its efficiency in cytogenetic diagnosis of acute 
      leukemia. METHODS: The original multiple-probe FISH techniques were optimized by 
      adjusting the cell density and adding a process of protease digestion. 
      Cytogenetic anomalies were detected in 141 patients with acute lymphocytic 
      leukemia (ALL) or acute myeloid leukemia/ myelodysplastic syndromes (AML/MDS) 
      using the modified technique, and 35 of the patients were also examined using the 
      original technique. The successful detection rate and positive site detection 
      rate were compared between the modified and original techniques. RESULTS: 
      Modification of the pre-coated multiple-probe FISH technique resulted in an 
      significant increase of the successful detection rate (from 85.3% to 100%) and 
      the positive site detection rate (from 5.1% to 8.6%) in ALL patients; in AML/MDS 
      patients, the successful detection rate was significantly improved from 67.4% to 
      99.8% and the positive site detection rate from 3.5% to 6.0% (P<0.01). 
      CONCLUSION: The modified pre-coated multiple-probe FISH technique can 
      significantly increase the diagnostic efficiency of cytogenetic abnormalities in 
      leukemic patients.
FAU - Cao, Rui
AU  - Cao R
AD  - Department of Hematology, Southern Medical University, Guangzhou, China. 
      zhujiangcaorui@163.com
FAU - Song, Lanlin
AU  - Song L
FAU - Wu, Fuqun
AU  - Wu F
FAU - Liao, Libin
AU  - Liao L
FAU - Zuo, Yuan
AU  - Zuo Y
FAU - Liu, Xiaoli
AU  - Liu X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
SB  - IM
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*diagnosis/genetics
MH  - Myelodysplastic Syndromes/*diagnosis/genetics
EDAT- 2012/10/19 06:00
MHDA- 2013/07/26 06:00
CRDT- 2012/10/19 06:00
PHST- 2012/10/19 06:00 [entrez]
PHST- 2012/10/19 06:00 [pubmed]
PHST- 2013/07/26 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2012 Oct;32(10):1457-60.