PMID- 23077169
OWN - NLM
STAT- MEDLINE
DCOM- 20130514
LR  - 20151119
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 87
IP  - 6
DP  - 2012 Jun
TI  - Hepatocyte growth factor is a mouse fetal Leydig cell terminal differentiation 
      factor.
PG  - 146
LID - 10.1095/biolreprod.112.104638 [doi]
AB  - The hepatocyte growth factor (HGF) is a pleiotropic cytokine and a well-known 
      regulator of mouse embryonic organogenesis. In previous papers, we have shown the 
      expression pattern of HGF and its receptor, C-MET, during the different stages of 
      testis prenatal development. We demonstrated that C-MET is expressed in fetal 
      Leydig cells (FLCs) and that HGF stimulates testosterone secretion in organ 
      culture of late fetal testes. In the present study, we analyzed the proliferation 
      rate, apoptotic index, and differentiation of FLCs in testicular organ culture of 
      17.5 days postcoitum (17.5 dpc) embryos to clarify the physiological role of HGF 
      in late testis organogenesis. Based on our data, we conclude the following: 1) 
      HGF acts as an antiapoptotic factor that is able to reduce the number of 
      apoptotic FLCs and testicular caspase-3 active fragment; 2) HGF does not affect 
      FLC proliferation; 3) HGF significantly increases expression of insulin-like 3 
      (INSL3), a marker of Leydig cell terminal differentiation, without affecting 
      3beta-hydroxysteroid dehydrogenase (3betaHSD) expression; 4) HGF significantly 
      decreases the expression of nestin, a marker of Leydig cell progenitors; and 5) 
      HGF significantly increases the number of fully developed FLCs. Taken together, 
      these observations demonstrate that HGF is able to act in vitro as a survival and 
      differentiation factor in FLC population.
FAU - Ricci, Giulia
AU  - Ricci G
AD  - Department of Experimental Medicine, Histology and Embryology Laboratory, School 
      of Medicine, Second University of Naples, Naples, Italy. giulia.ricci@unina2.it
FAU - Guglielmo, Maria Cristina
AU  - Guglielmo MC
FAU - Caruso, Maria
AU  - Caruso M
FAU - Ferranti, Francesca
AU  - Ferranti F
FAU - Canipari, Rita
AU  - Canipari R
FAU - Galdieri, Michela
AU  - Galdieri M
FAU - Catizone, Angela
AU  - Catizone A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121221
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Biomarkers)
RN  - 0 (HGF protein, mouse)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nes protein, mouse)
RN  - 0 (Nestin)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - Caspase 3/metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Intermediate Filament Proteins/metabolism
MH  - Leydig Cells/*cytology/enzymology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Nerve Tissue Proteins/metabolism
MH  - Nestin
MH  - Organ Culture Techniques
MH  - *Organogenesis
MH  - Peptide Fragments/metabolism
MH  - Proto-Oncogene Proteins c-met/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - *Signal Transduction
MH  - Testis/cytology/*embryology/metabolism
EDAT- 2012/10/19 06:00
MHDA- 2013/05/15 06:00
CRDT- 2012/10/19 06:00
PHST- 2012/10/19 06:00 [entrez]
PHST- 2012/10/19 06:00 [pubmed]
PHST- 2013/05/15 06:00 [medline]
AID - biolreprod.112.104638 [pii]
AID - 10.1095/biolreprod.112.104638 [doi]
PST - epublish
SO  - Biol Reprod. 2012 Dec 21;87(6):146. doi: 10.1095/biolreprod.112.104638. Print 
      2012 Jun.