PMID- 23078630
OWN - NLM
STAT- MEDLINE
DCOM- 20150721
LR  - 20240109
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 14
IP  - 5
DP  - 2012 Oct 19
TI  - Interleukin-29 modulates proinflammatory cytokine production in synovial 
      inflammation of rheumatoid arthritis.
PG  - R228
LID - 10.1186/ar4067 [doi]
AB  - INTRODUCTION: The immunoregulatory function of interleukin (IL)-29 has recently 
      been recognized. However, little is known about the involvement of IL-29 in the 
      pathogenesis of rheumatoid arthritis (RA). This study aimed to examine the 
      expression profiles of IL-29 in blood, synovial fluid (SF) and synovium in RA 
      patients and investigate the effect of IL-29 on cytokines production in RA 
      synovial fibroblasts. METHODS: The transcript levels of IL-29 and its specific 
      receptor IL-28Ralpha in peripheral blood mononuclear cells (PBMC) and synovium were 
      determined by real-time reverse transcription-polymerase chain reaction 
      (real-time PCR). The concentrations of IL-29 in serum and synovial fluid (SF) 
      were quantified by enzyme-linked immunoassay (ELISA), and the correlation of 
      serum IL-29 levels with disease activity in RA patients was investigated. 
      Furthermore, the expression of IL-29 in RA synovium was examined by 
      immunohistochemistry and double immunofluorescence analysis. Finally, the 
      expression of IL-6, IL-8, IL-10, IL-17 and matrix metalloproteinase-3 (MMP-3) in 
      synovial fibroblasts upon IL-29 stimulation was determined by real-time PCR. 
      RESULTS: IL-29 and IL-28Ralpha mRNA expression in PBMC was significantly increased in 
      patients with RA compared with healthy controls (HC). The serum levels of 
      circulating IL-29 were higher in RA than those in HC. Increased IL-29 levels were 
      detected in RA SF when compared with osteoarthritis (OA) SF. However, serum IL-29 
      levels showed no significant correlation with RA disease activity. IL-29 was 
      mostly expressed in the lining region of RA synovium. Moreover, IL-29 was 
      expressed predominately in synovial macrophages and fibroblasts. RA synovial 
      fibroblasts exposed to IL-29 specifically upregulated IL-6, -8 and MMP-3 but 
      downregulated IL-10. CONCLUSIONS: The findings in the present study indicate, for 
      the first time, that IL-29 is dysregulated in patients with RA, which may 
      contribute to the RA pathogenesis via inducing the production of proinflammatory 
      cytokines, chemokines or matrix metalloproteinases in synovial fibroblasts.
FAU - Wang, Fang
AU  - Wang F
FAU - Xu, Lingxiao
AU  - Xu L
FAU - Feng, Xiaoke
AU  - Feng X
FAU - Guo, Dunming
AU  - Guo D
FAU - Tan, Wenfeng
AU  - Tan W
FAU - Zhang, Miaojia
AU  - Zhang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121019
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Cytokines)
RN  - 0 (interferon-lambda, human)
RN  - 0 (Interleukins)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (interleukin 28alpha receptor)
RN  - 9008-11-1 (Interferons)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthritis, Rheumatoid/*metabolism/pathology
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Female
MH  - Fibroblasts/drug effects/metabolism/pathology
MH  - Humans
MH  - Interferons
MH  - Interleukins/*metabolism/pharmacology
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Middle Aged
MH  - Osteoarthritis/metabolism/pathology
MH  - Receptors, Cytokine/metabolism
MH  - Severity of Illness Index
MH  - Synovial Fluid/metabolism
MH  - Synovial Membrane/*metabolism
MH  - Synovitis/*metabolism
MH  - Young Adult
PMC - PMC3580539
EDAT- 2012/10/20 06:00
MHDA- 2015/07/22 06:00
PMCR- 2012/10/19
CRDT- 2012/10/20 06:00
PHST- 2012/06/28 00:00 [received]
PHST- 2012/10/09 00:00 [accepted]
PHST- 2012/10/20 06:00 [entrez]
PHST- 2012/10/20 06:00 [pubmed]
PHST- 2015/07/22 06:00 [medline]
PHST- 2012/10/19 00:00 [pmc-release]
AID - ar4067 [pii]
AID - 10.1186/ar4067 [doi]
PST - epublish
SO  - Arthritis Res Ther. 2012 Oct 19;14(5):R228. doi: 10.1186/ar4067.