PMID- 23082472
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20211203
IS  - 1110-4902 (Print)
VI  - 18
IP  - 2
DP  - 2011
TI  - Detection of bacterial DNA in peripheral blood mononuclear cells of patients with 
      reactive arthritis.
PG  - 67-76
AB  - Reactive arthritis (ReA) is an infection-induced systemic illness characterized 
      by an aseptic inflammatory joint involvement and occurring in genetically 
      predisposed patients with a bacterial infection localized in a distant organ or 
      system. We evaluated the possible role of bacterial infection in the etiology of 
      reactive arthritis by testing peripheral blood monocytes (PBMCs) for the presence 
      of prokaryotic 16S ribosomal RNA genes which are known as 16S rDNA. PBMCs were 
      isolated from 40 patients with ReA and 10 healthy controls. Clinical, laboratory 
      and radiological evaluations were carried out for all patients and controls. 
      Bacterial DNA was extracted from the PBMCs and subjected to PCR amplification of 
      the 16S rDNA gene followed by DNA sequencing and database comparative analysis. 
      Bacterial DNA was detected in 16/40 (40%) patients and 3/10 (30%) healthy 
      controls. No significant difference was detected between the PCR +ve and -ve 
      groups of patients as regards arthritis, arthralgia, sacroiliitis, low back pain 
      and enthesopathies (P>0.05), while a significant difference was detected in the 
      PCR +ve females with gynaecological infection (P<0.05). A significant difference 
      of the pattern of arthritis was also observed between the two groups of patients. 
      Comparative analysis of 7 16S rDNA sequences from patients and controls using 
      Basic Local Alignment Search Tool (BLAST) and the National Center for 
      Biotechnology Information (NCB1) database revealed high % similarity with 
      potential pathogens and nonpathogenic bacteria. Further studies are needed to 
      establish the exact role of these organisms in the pathogenesis of ReA.
FAU - Ibrahiem, Bahaa E
AU  - Ibrahiem BE
AD  - Department of Internal Medicine, Faculty of Medicine (for girls), Al-Azhar 
      University, Cairo, Egypt.
FAU - El Haliem, Naglaa F Abd
AU  - El Haliem NF
FAU - El-Khouly, Nashwa
AU  - El-Khouly N
FAU - El-Bazz, Walaa F
AU  - El-Bazz WF
FAU - Al-Raoof, Mona Abd
AU  - Al-Raoof MA
FAU - Said, Zinab N
AU  - Said ZN
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
RN  - 0 (DNA, Bacterial)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Arthritis, Reactive/*blood/immunology/*microbiology
MH  - Bacterial Infections/*blood/immunology/microbiology
MH  - Chi-Square Distribution
MH  - DNA, Bacterial/*blood/chemistry/genetics
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/microbiology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Prohibitins
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Young Adult
EDAT- 2011/01/01 00:00
MHDA- 2012/11/14 06:00
CRDT- 2012/10/23 06:00
PHST- 2012/10/23 06:00 [entrez]
PHST- 2011/01/01 00:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
PST - ppublish
SO  - Egypt J Immunol. 2011;18(2):67-76.