PMID- 23084054
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 751
DP  - 2012 Nov 2
TI  - Liquid-liquid-solid microextraction and detection of nerve agent simulants by 
      on-membrane Fourier transform infrared spectroscopy.
PG  - 71-8
LID - S0003-2670(12)01340-2 [pii]
LID - 10.1016/j.aca.2012.09.013 [doi]
AB  - A coupling of novel liquid-liquid-solid microextraction (LLSME) technique based 
      on porous hydrophobic membrane and Fourier-transform infrared spectroscopy has 
      been presented for the detection, identification and quantification of markers 
      and simulants of nerve agents. Two isomers O,O'-dihexyl methylphosphonate (DHMP) 
      and O,O'-dipentyl isopropylphosphonate (DPIPP) were chosen as model analytes for 
      the study. In the present technique, organic phase was immobilised within the 
      pores of membrane after fixing it in an assembly, which was then immersed into 
      aqueous sample of target analytes for extraction. The analytes were directly 
      determined on the surface of membrane by FTIR spectroscopy without elution. On 
      comparison with solid phase microextraction (SPME), LLSME was found to be much 
      more efficient. The method was optimised and quantitative analyses were performed 
      using calibration curves obtained via Beer's law and employing processing of 
      spectra obtained, via a multivariate calibration technique partial least square 
      (PLS). Relative standard deviations (RSDs) for intraday repeatability and 
      interday reproducibility were found to be in the range of 0.20-0.50% and 
      0.20-0.60%, respectively. Limit of detection (LOD) was achieved up to 15 ng 
      mL(-1). Applicability of the method was tested with an unknown real sample 
      obtained in an international official proficiency test (OPT).
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Garg, Prabhat
AU  - Garg P
AD  - Vertox Laboratory, Defence Research & Development Establishment, Gwalior 474002, 
      India.
FAU - Purohit, Ajay
AU  - Purohit A
FAU - Tak, Vijay K
AU  - Tak VK
FAU - Kumar, Ajeet
AU  - Kumar A
FAU - Dubey, D K
AU  - Dubey DK
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20120918
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Chemical Warfare Agents)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Organophosphorus Compounds)
RN  - 059QF0KO0R (Water)
RN  - 329W4YM10Z (methylphosphonic acid)
SB  - IM
MH  - Chemical Warfare Agents/*analysis/isolation & purification
MH  - Equipment Design
MH  - Limit of Detection
MH  - Liquid Phase Microextraction/*instrumentation
MH  - Membranes, Artificial
MH  - Organophosphorus Compounds/*analysis/isolation & purification
MH  - Reproducibility of Results
MH  - Spectroscopy, Fourier Transform Infrared/*instrumentation
MH  - Water/*analysis
EDAT- 2012/10/23 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/10/23 06:00
PHST- 2012/06/13 00:00 [received]
PHST- 2012/09/04 00:00 [revised]
PHST- 2012/09/11 00:00 [accepted]
PHST- 2012/10/23 06:00 [entrez]
PHST- 2012/10/23 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - S0003-2670(12)01340-2 [pii]
AID - 10.1016/j.aca.2012.09.013 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2012 Nov 2;751:71-8. doi: 10.1016/j.aca.2012.09.013. Epub 2012 
      Sep 18.