PMID- 23085275
OWN - NLM
STAT- MEDLINE
DCOM- 20130201
LR  - 20211203
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 512
IP  - 2
DP  - 2013 Jan 10
TI  - Mechanistic target of rapamycin in common carp: cDNA cloning, characterization, 
      and tissue expression.
PG  - 566-72
LID - S0378-1119(12)00955-9 [pii]
LID - 10.1016/j.gene.2012.08.006 [doi]
AB  - The mechanistic target of rapamycin (mTOR) plays a key role in growth and 
      development. In the present study, a cDNA encoding mTOR protein was identified 
      from common carp Cyprinus carpio muscle. The open reading frame of this cDNA 
      encodes 2515 deduced amino acid residues that showed high sequence similarity 
      with its zebrafish Danio rerio counterparts. Bioinformatic analysis showed that 
      the protein belongs to the PI-3 kinase family. The putative protein has FAT, FRB, 
      PI3Kc, and FATC domains, which are highly conserved among the vertebrate 
      orthologs. Real-time quantitative PCR analysis revealed that the abundance of 
      mTOR mRNA was the highest in the heart at 18-31 g and muscle at 31-75g of common 
      carp. As the common carp grew (18-40 g), the mTOR expression gradually decreased 
      in the hepatopancreas and heart, but increased in the muscle, hind kidney, 
      spleen, gill, head kidney, foregut, midgut, and hindgut. Then the mTOR expression 
      kept a constant in all examined tissues during the common carp grew (40-75g). The 
      present study identified the mTOR gene and determined its gene expression profile 
      in various tissues of the common carp with body weight increased. These findings 
      will help better understand the biological role of mTOR in the juvenile fish.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Jiang, Jun
AU  - Jiang J
AD  - Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
FAU - Feng, Lin
AU  - Feng L
FAU - Liu, Yang
AU  - Liu Y
FAU - Jiang, Wei-Dan
AU  - Jiang WD
FAU - Hu, Kai
AU  - Hu K
FAU - Li, Shu-Hong
AU  - Li SH
FAU - Zhou, Xiao-Qiu
AU  - Zhou XQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121022
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Carps/genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - *Fish Proteins/biosynthesis/genetics
MH  - Gene Expression Regulation/*physiology
MH  - Organ Specificity/physiology
MH  - Sequence Homology, Amino Acid
MH  - *TOR Serine-Threonine Kinases/biosynthesis/genetics
MH  - Zebrafish/genetics/metabolism
EDAT- 2012/10/23 06:00
MHDA- 2013/02/05 06:00
CRDT- 2012/10/23 06:00
PHST- 2012/03/22 00:00 [received]
PHST- 2012/07/04 00:00 [revised]
PHST- 2012/08/02 00:00 [accepted]
PHST- 2012/10/23 06:00 [entrez]
PHST- 2012/10/23 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
AID - S0378-1119(12)00955-9 [pii]
AID - 10.1016/j.gene.2012.08.006 [doi]
PST - ppublish
SO  - Gene. 2013 Jan 10;512(2):566-72. doi: 10.1016/j.gene.2012.08.006. Epub 2012 Oct 
      22.