PMID- 23085949
OWN - NLM
STAT- MEDLINE
DCOM- 20130731
LR  - 20211021
IS  - 2042-0226 (Electronic)
IS  - 1672-7681 (Print)
IS  - 1672-7681 (Linking)
VI  - 9
IP  - 6
DP  - 2012 Nov
TI  - The equivalents of human blood and spleen dendritic cell subtypes can be 
      generated in vitro from human CD34(+) stem cells in the presence of fms-like 
      tyrosine kinase 3 ligand and thrombopoietin.
PG  - 446-54
LID - 10.1038/cmi.2012.48 [doi]
AB  - Dendritic cells (DCs) are immune cells specialized to capture, process and 
      present antigen to T cells in order to initiate an appropriate adaptive immune 
      response. The study of mouse DC has revealed a heterogeneous population of cells 
      that differ in their development, surface phenotype and function. The study of 
      human blood and spleen has shown the presence of two subsets of conventional DC 
      including the CD1b/c(+) and CD141(+)CLEC9A(+) conventional DC (cDC) and a 
      plasmacytoid DC (pDC) that is CD304(+)CD123(+). Studies on these subpopulations 
      have revealed phenotypic and functional differences that are similar to those 
      described in the mouse. In this study, the three DC subsets have been generated 
      in vitro from human CD34(+) precursors in the presence of fms-like tyrosine 
      kinase 3 ligand (Flt3L) and thrombopoietin (TPO). The DC subsets so generated, 
      including the CD1b/c(+) and CLEC9A(+) cDCs and CD123(+) pDCs, were largely 
      similar to their blood and spleen counterparts with respect to surface phenotype, 
      toll-like receptor and transcription factor expression, capacity to stimulate T 
      cells, cytokine secretion and cross-presentation of antigens. This system may be 
      utilized to study aspects of DC development and function not possible in vivo.
FAU - Proietto, A I
AU  - Proietto AI
AD  - Molecular Immunology Division, The Walter and Eliza Hall Institute of Medical 
      Research, Parkville, Vic., Australia. proietto@wehi.edu.au
FAU - Mittag, D
AU  - Mittag D
FAU - Roberts, A W
AU  - Roberts AW
FAU - Sprigg, N
AU  - Sprigg N
FAU - Wu, L
AU  - Wu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121022
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
RN  - 0 (Antigens, CD34)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Proteins)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factors)
RN  - 0 (flt3 ligand protein)
RN  - 9014-42-0 (Thrombopoietin)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Blood Cells/*cytology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/drug effects/immunology
MH  - Cell Culture Techniques/*methods
MH  - Cell Shape/drug effects
MH  - Cross-Priming/drug effects
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Hematopoietic Stem Cells/*cytology/drug effects/metabolism
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Membrane Proteins/*pharmacology
MH  - Mice
MH  - Monocytes/cytology/drug effects/metabolism
MH  - Phenotype
MH  - Spleen/*cytology
MH  - Thrombopoietin/*pharmacology
MH  - Toll-Like Receptors/metabolism
MH  - Transcription Factors/metabolism
PMC - PMC4002222
EDAT- 2012/10/23 06:00
MHDA- 2013/08/01 06:00
PMCR- 2012/11/01
CRDT- 2012/10/23 06:00
PHST- 2012/10/23 06:00 [entrez]
PHST- 2012/10/23 06:00 [pubmed]
PHST- 2013/08/01 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - cmi201248 [pii]
AID - 10.1038/cmi.2012.48 [doi]
PST - ppublish
SO  - Cell Mol Immunol. 2012 Nov;9(6):446-54. doi: 10.1038/cmi.2012.48. Epub 2012 Oct 
      22.