PMID- 23086220
OWN - NLM
STAT- MEDLINE
DCOM- 20130607
LR  - 20211021
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 193
IP  - 1
DP  - 2013 Jan
TI  - Dispensable, redundant, complementary, and cooperative roles of dopamine, 
      octopamine, and serotonin in Drosophila melanogaster.
PG  - 159-76
LID - 10.1534/genetics.112.142042 [doi]
AB  - To investigate the regulation of Drosophila melanogaster behavior by biogenic 
      amines, we have exploited the broad requirement of the vesicular monoamine 
      transporter (VMAT) for the vesicular storage and exocytotic release of all 
      monoamine neurotransmitters. We used the Drosophila VMAT (dVMAT) null mutant to 
      globally ablate exocytotic amine release and then restored DVMAT activity in 
      either individual or multiple aminergic systems, using transgenic rescue 
      techniques. We find that larval survival, larval locomotion, and female fertility 
      rely predominantly on octopaminergic circuits with little apparent input from the 
      vesicular release of serotonin or dopamine. In contrast, male courtship and 
      fertility can be rescued by expressing DVMAT in octopaminergic or dopaminergic 
      neurons, suggesting potentially redundant circuits. Rescue of major aspects of 
      adult locomotion and startle behavior required octopamine, but a complementary 
      role was observed for serotonin. Interestingly, adult circadian behavior could 
      not be rescued by expression of DVMAT in a single subtype of aminergic neurons, 
      but required at least two systems, suggesting the possibility of unexpected 
      cooperative interactions. Further experiments using this model will help 
      determine how multiple aminergic systems may contribute to the regulation of 
      other behaviors. Our data also highlight potential differences between behaviors 
      regulated by standard exocytotic release and those regulated by other mechanisms.
FAU - Chen, Audrey
AU  - Chen A
AD  - Department of Neurobiology, University of California, Los Angeles, CA 90095, USA.
FAU - Ng, Fanny
AU  - Ng F
FAU - Lebestky, Tim
AU  - Lebestky T
FAU - Grygoruk, Anna
AU  - Grygoruk A
FAU - Djapri, Christine
AU  - Djapri C
FAU - Lawal, Hakeem O
AU  - Lawal HO
FAU - Zaveri, Harshul A
AU  - Zaveri HA
FAU - Mehanzel, Filmon
AU  - Mehanzel F
FAU - Najibi, Rod
AU  - Najibi R
FAU - Seidman, Gabriel
AU  - Seidman G
FAU - Murphy, Niall P
AU  - Murphy NP
FAU - Kelly, Rachel L
AU  - Kelly RL
FAU - Ackerson, Larry C
AU  - Ackerson LC
FAU - Maidment, Nigel T
AU  - Maidment NT
FAU - Jackson, F Rob
AU  - Jackson FR
FAU - Krantz, David E
AU  - Krantz DE
LA  - eng
GR  - R01 ES015747/ES/NIEHS NIH HHS/United States
GR  - GM07185/GM/NIGMS NIH HHS/United States
GR  - T32 HD049341/HD/NICHD NIH HHS/United States
GR  - T32 ES015457/ES/NIEHS NIH HHS/United States
GR  - R25 GM055052/GM/NIGMS NIH HHS/United States
GR  - T32 GM007185/GM/NIGMS NIH HHS/United States
GR  - R01 HL59873/HL/NHLBI NIH HHS/United States
GR  - P30 NS047243/NS/NINDS NIH HHS/United States
GR  - R01 HL059873/HL/NHLBI NIH HHS/United States
GR  - R01 MH076900/MH/NIMH NIH HHS/United States
GR  - R01 NS065900/NS/NINDS NIH HHS/United States
GR  - P01 ES016732/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20121019
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Drosophila Proteins)
RN  - 0 (Neurotransmitter Agents)
RN  - 14O50WS8JD (Octopamine)
RN  - 333DO1RDJY (Serotonin)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Circadian Rhythm/genetics
MH  - Dopamine/*metabolism
MH  - Drosophila Proteins/genetics/metabolism
MH  - Drosophila melanogaster/genetics/*metabolism
MH  - Female
MH  - Larva/metabolism
MH  - Locomotion
MH  - Male
MH  - Mutation
MH  - Neurotransmitter Agents/*metabolism
MH  - Octopamine/*metabolism
MH  - Reflex, Startle/genetics
MH  - Reproduction
MH  - Serotonin/*metabolism
MH  - Sexual Behavior, Animal
PMC - PMC3527243
EDAT- 2012/10/23 06:00
MHDA- 2013/06/08 06:00
PMCR- 2014/01/01
CRDT- 2012/10/23 06:00
PHST- 2012/10/23 06:00 [entrez]
PHST- 2012/10/23 06:00 [pubmed]
PHST- 2013/06/08 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - genetics.112.142042 [pii]
AID - 142042 [pii]
AID - 10.1534/genetics.112.142042 [doi]
PST - ppublish
SO  - Genetics. 2013 Jan;193(1):159-76. doi: 10.1534/genetics.112.142042. Epub 2012 Oct 
      19.