PMID- 23087365
OWN - NLM
STAT- MEDLINE
DCOM- 20130227
LR  - 20181202
IS  - 1097-6817 (Electronic)
IS  - 0194-5998 (Linking)
VI  - 148
IP  - 1
DP  - 2013 Jan
TI  - Expression and significance of hypoxia-inducible factor-1alpha and survivin in 
      laryngeal carcinoma tissue and cells.
PG  - 75-81
LID - 10.1177/0194599812464759 [doi]
AB  - OBJECTIVE: The aim was to evaluate hypoxia-inducible factor-1alpha (HIF-1alpha) and 
      survivin expression in laryngeal squamous cell carcinoma (LSCC) tissues and cell 
      lines and to investigate whether HIF-1alpha has an effect on the regulation of 
      survivin gene expression in LSCC cells under hypoxia. STUDY DESIGN: Prospective, 
      observational. SETTING: Shanghai Jiaotong University Affiliated First People's 
      Hospital. SUBJECTS AND METHODS: The expression of HIF-1alpha and survivin protein in 
      human LSCC tissues was analyzed by immunohistochemistry. HIF-1alpha and survivin gene 
      expression levels in Hep-2 cells were detected by real-time quantitative reverse 
      transcription polymerase chain reaction (RT-PCR) and Western blot under normoxic 
      or hypoxic conditions. In hypoxic cells, HIF-1alpha expression was inhibited by RNA 
      interference. RESULTS: HIF-1alpha and survivin were both highly expressed in LSCC 
      tissues and significantly related to the clinical stage and lymph node metastasis 
      (P < .05). Meanwhile, a positive correlation existed between HIF-1alpha and survivin 
      expression (r = 0.456, P < .01). In LSCC cells, HIF-1alpha and survivin expression 
      were obviously upregulated in response to hypoxia (P < .05). The downregulation 
      of HIF-1alpha expression dramatically decreased survivin gene expression in hypoxic 
      cells (P < .05). CONCLUSION: HIF-1alpha could be considered as an important regulator 
      for the upregulation of survivin gene expression induced by hypoxia in LSCC 
      cells, and both proteins could be regarded as 2 key predictors of malignant 
      progression and metastasis of LSCC.
FAU - Li, Da-wei
AU  - Li DW
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye and ENT 
      Hospital of Fudan University, Shanghai, China.
FAU - Zhou, Liang
AU  - Zhou L
FAU - Jin, Bin
AU  - Jin B
FAU - Xie, Jin
AU  - Xie J
FAU - Dong, Pin
AU  - Dong P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121019
PL  - England
TA  - Otolaryngol Head Neck Surg
JT  - Otolaryngology--head and neck surgery : official journal of American Academy of 
      Otolaryngology-Head and Neck Surgery
JID - 8508176
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Survivin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Analysis of Variance
MH  - Biomarkers, Tumor/analysis
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology/surgery
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics/metabolism
MH  - Immunohistochemistry
MH  - Inhibitor of Apoptosis Proteins/*genetics/metabolism
MH  - Laryngeal Neoplasms/*genetics/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Survival Rate
MH  - Survivin
MH  - Tissue Embedding
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
EDAT- 2012/10/23 06:00
MHDA- 2013/02/28 06:00
CRDT- 2012/10/23 06:00
PHST- 2012/10/23 06:00 [entrez]
PHST- 2012/10/23 06:00 [pubmed]
PHST- 2013/02/28 06:00 [medline]
AID - 0194599812464759 [pii]
AID - 10.1177/0194599812464759 [doi]
PST - ppublish
SO  - Otolaryngol Head Neck Surg. 2013 Jan;148(1):75-81. doi: 10.1177/0194599812464759. 
      Epub 2012 Oct 19.