PMID- 23089247
OWN - NLM
STAT- MEDLINE
DCOM- 20130820
LR  - 20131121
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 181
DP  - 2013 Jan 15
TI  - Estrogenic regulation of Kiss1 mRNA variants in Hatano rats.
PG  - 246-53
LID - S0016-6480(12)00409-1 [pii]
LID - 10.1016/j.ygcen.2012.10.004 [doi]
AB  - Differences in reproductive endocrinology distinguish Hatano high-avoidance 
      animals (HAA) from low-avoidance animals (LAA). Compared to HAA rats, female LAA 
      rats secrete low levels of basal luteinizing hormone (LH) and a reduced LH surge. 
      To investigate the underlying cause of the differences between the two strains, 
      levels of the following mRNAs were measured in the hypothalamus of intact and 
      ovariectomized (OVX) females treated with vehicle control or estradiol-17beta (E2): 
      gonadotropin-releasing hormone (Gnrh), newly isolated rat kisspeptin (Kiss)1 mRNA 
      variant-1 (Kiss1V1) and variant-2 (Kiss1V2) and estrogen receptor (Er) alpha. In 
      OVX-HAA rats, the levels of Gnrh mRNA in the preoptic area (POA) 30 h after E2 
      treatment were significantly higher than in OVX-LAA rats. For HAA rats, the 
      levels of Kiss1V1 and Kiss1V2 mRNA in the anteroventral periventricular nucleus 
      (AVPV) were significantly higher in the E2-treated group than in the 
      vehicle-treated group. In the arcuate nucleus (Arc) of HAA rats, Kiss1V1 and 
      Kiss1V2 expression was significantly lower in E2-treated females compared to 
      vehicle-treated females. Kiss1V2 expression was significantly higher than Kiss1V1 
      expression in intact HAA rats. In E2-treated OVX-LAA rats, there were no changes 
      in the expression levels of Gnrh, Kiss1V1 or Kiss1V2. In intact LAA rats, no 
      differences were observed in the expression levels of Kiss1V1 or Kiss1V2 in the 
      AVPV, but the expression levels of these mRNAs in the Arc were significantly 
      lower in E2-treated OVX-LAA rats. Additionally, no strain differences were 
      observed for Eralpha mRNA expression in either the AVPV or Arc. These results 
      indicate that the failure of estrogenic regulation of GnRH neurons in the POA and 
      of kisspeptin neurons in the AVPV of LAA rats causes low LH secretion and reduced 
      reproductive function.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Horii, Yasuyuki
AU  - Horii Y
AD  - The United Graduate School of Veterinary Sciences, Gifu University, Gifu 
      501-1193, Japan.
FAU - Dalpatadu, Sandun L
AU  - Dalpatadu SL
FAU - Soga, Tomoko
AU  - Soga T
FAU - Ohta, Ryo
AU  - Ohta R
FAU - Watanabe, Gen
AU  - Watanabe G
FAU - Taya, Kazuyoshi
AU  - Taya K
FAU - Parhar, Ishwar S
AU  - Parhar IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Kiss1 protein, rat)
RN  - 0 (Kisspeptins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - Estrous Cycle/drug effects/genetics
MH  - Female
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Kisspeptins/*genetics
MH  - Luteinizing Hormone/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Binding/drug effects/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Reproduction/drug effects/genetics
EDAT- 2012/10/24 06:00
MHDA- 2013/08/21 06:00
CRDT- 2012/10/24 06:00
PHST- 2012/09/26 00:00 [received]
PHST- 2012/10/09 00:00 [revised]
PHST- 2012/10/10 00:00 [accepted]
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0016-6480(12)00409-1 [pii]
AID - 10.1016/j.ygcen.2012.10.004 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2013 Jan 15;181:246-53. doi: 10.1016/j.ygcen.2012.10.004. 
      Epub 2012 Oct 23.