PMID- 23089668
OWN - NLM
STAT- MEDLINE
DCOM- 20130321
LR  - 20190727
IS  - 1349-3329 (Electronic)
IS  - 0040-8727 (Linking)
VI  - 228
IP  - 3
DP  - 2012 Nov
TI  - Low NRF2 mRNA expression in spermatozoa from men with low sperm motility.
PG  - 259-66
AB  - Antioxidant genes and enzymes play important roles in human spermatogenesis. 
      Although low levels of antioxidant enzyme expression are associated with poor 
      sperm quality, it is not clear whether mRNA expression of antioxidant genes is 
      lower in these men than in normozoospermic men. In this study, 55 
      asthenozoospermic and oligoasthenozoospermic patients and 65 controls were 
      recruited. Quantitative real-time reverse transcription PCR was performed and the 
      abundance of mRNA of four antioxidant genes known to be important to 
      spermatogenesis were evaluated. These genes were nuclear factor erythroid 
      2-related factor 2 (NRF2), catalase (CAT), glutathione S-transferase Mu 1 
      (GSTM1), and superoxide dismutase isoenzyme 2 (SOD2). Results showed the level of 
      NRF2 mRNA expression to be significantly lower in patients than in controls (P < 
      0.001), but no statistically significant difference in the level of SOD2, CAT, or 
      GSTM1 gene expression was observed between the two groups. A significant 
      correlation was observed between the level of NRF2 mRNA expression and specific 
      sperm function parameters, including concentration, progressive motility, 
      immotility, vitality, and morphology (all P < 0.01). NRF2 expression was also 
      found to be associated with seminal SOD activity and mRNA levels of the CAT and 
      SOD2 genes (all P < 0.05). Therefore, our data demonstrated that the level of 
      NRF2 mRNA expression is significantly lower in human males with low sperm 
      motility and correlated with specific sperm function parameters. This suggests 
      that NRF2 is important to spermatogenesis and may serve as a useful biomarker in 
      the prediction of male infertility.
FAU - Chen, Kang
AU  - Chen K
AD  - Key Laboratory of Reproduction and Genetics of Guangdong Higher Education 
      Institutes, the Third Affiliated Hospital of Guangzhou Medical University, 
      Guangzhou, PR China.
FAU - Mai, Zixin
AU  - Mai Z
FAU - Zhou, Yulin
AU  - Zhou Y
FAU - Gao, Xingcheng
AU  - Gao X
FAU - Yu, Bolan
AU  - Yu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Biomarkers)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Asthenozoospermia/diagnosis/*genetics/metabolism
MH  - Biomarkers/analysis/metabolism
MH  - Case-Control Studies
MH  - Down-Regulation/genetics
MH  - Gene Expression
MH  - Humans
MH  - Infertility, Male/diagnosis/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Prognosis
MH  - RNA, Messenger/metabolism
MH  - Semen Analysis/methods
MH  - Sperm Motility/genetics
MH  - Spermatogenesis/genetics
MH  - Spermatozoa/*metabolism
EDAT- 2012/10/24 06:00
MHDA- 2013/03/22 06:00
CRDT- 2012/10/24 06:00
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/03/22 06:00 [medline]
AID - DN/JST.JSTAGE/tjem/228.259 [pii]
AID - 10.1620/tjem.228.259 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 2012 Nov;228(3):259-66. doi: 10.1620/tjem.228.259.