PMID- 23090649
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 405
IP  - 1
DP  - 2013 Jan
TI  - Covalent attachment of functionalized cardiolipin on a biosensor gold surface 
      allows repetitive measurements of anticardiolipin antibodies in serum.
PG  - 275-85
LID - 10.1007/s00216-012-6467-8 [doi]
AB  - Antiphospholipid antibodies (aPL) are a relevant serological indicator of 
      antiphospholipid syndrome (APS). A solid-state surface with covalently bound 
      omega-amine-functionalized cardiolipin was established and the binding of 
      beta2-glycoprotein I (beta2-GPI) was investigated either by use of surface plasmon 
      resonance (SPR) biosensor, by electrically switchable DNA interfaces 
      (switchSENSE) and by scanning tunneling microscopy (STM). STM could clearly 
      visualize the attachment of beta2-GPI to the cardiolipin surface. Using the 
      switchSENSE sensor, beta2-GPI as specific ligand could be identified by increased 
      hydrodynamic friction. The binding of anti-cardiolipin antibodies (aCL) was 
      detected against the omega-amine-functionalized cardiolipin-modified SPR biosensor 
      (aCL biosensor) using sera from healthy donors, APS patients and syphilis 
      patients. Our results showed that the aCL biosensor is a much more sensitive 
      diagnostic device for APS patients compared to previous methods. The specificity 
      between beta2-GPI-dependent autoimmune- and beta2-GPI-independent infection-associated 
      types of aPLs was also studied and they can be distinguished by the different 
      binding kinetics and patterns.
FAU - Schlichtiger, Alice
AU  - Schlichtiger A
AD  - Institut fur Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar der 
      Technischen Universitat Munchen, Ismaninger Strasse 22, 81675 Munich, Germany. 
      schlichtiger@klinchem.med.tu-muenchen.de
FAU - Baier, Claudia
AU  - Baier C
FAU - Yin, Meng-Xin
AU  - Yin MX
FAU - Holmes, Andrew B
AU  - Holmes AB
FAU - Maruyama, Makiko
AU  - Maruyama M
FAU - Strasser, Ralf
AU  - Strasser R
FAU - Rant, Ulrich
AU  - Rant U
FAU - Thaler, Markus
AU  - Thaler M
FAU - Luppa, Peter B
AU  - Luppa PB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Antibodies, Anticardiolipin)
RN  - 0 (Cardiolipins)
RN  - 0 (Ligands)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 7440-57-5 (Gold)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies, Anticardiolipin/chemistry/*immunology
MH  - Antiphospholipid Syndrome/blood/immunology
MH  - *Biosensing Techniques
MH  - Cardiolipins/*chemistry
MH  - DNA/analysis
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Gold/*chemistry
MH  - Humans
MH  - Kinetics
MH  - Ligands
MH  - Microscopy, Scanning Tunneling/methods
MH  - Models, Chemical
MH  - Molecular Conformation
MH  - Surface Plasmon Resonance/methods
MH  - Surface Properties
MH  - Time Factors
MH  - beta 2-Glycoprotein I/chemistry
EDAT- 2012/10/24 06:00
MHDA- 2013/06/26 06:00
CRDT- 2012/10/24 06:00
PHST- 2012/06/29 00:00 [received]
PHST- 2012/09/28 00:00 [accepted]
PHST- 2012/09/18 00:00 [revised]
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - 10.1007/s00216-012-6467-8 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2013 Jan;405(1):275-85. doi: 10.1007/s00216-012-6467-8. Epub 
      2012 Oct 23.