PMID- 23090850
OWN - NLM
STAT- MEDLINE
DCOM- 20130528
LR  - 20121220
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 101
IP  - 2
DP  - 2013 Feb
TI  - Effect of BDNF-plasma-collagen matrix controlled delivery system on the behavior 
      of adult rats neural stem cells.
PG  - 599-606
LID - 10.1002/jbm.a.34331 [doi]
AB  - The neurogenesis amount in central nervous system (CNS) stimulated by the injury 
      or diseases is so small that neural stem cells (NSCs) cannot specifically 
      differentiate into the ideal phenotypes to repair the injured CNS. The 
      transplanted exogenous NSCs also have such problems as poor survival and 
      insufficient neuronal differentiation. In this study, the behavior of NSCs from 
      the spinal cord of adult rats was compared at the neurosphere level after the 
      respective addition of the brain-derived neurotrophic factor (BDNF) daily, the 
      BDNF-loaded plasma-collagen matrix, the plasma-collagen matrix alone, or the 
      defined medium alone. The results suggested that the BDNF, either in the control 
      release form or in the soluble form, initiated NSCs proliferation and 
      differentiation by activating receptors Trk B and p75NTR. BDNF also increased the 
      differentiation percentage of adult NSCs into neurons and supported the long-term 
      cell survival and growth. The BDNF was stably released by the plasma-collagen 
      matrix for up to 21 days. The plasma-collagen matrix alone showed its 
      biocompatibility with cells by facilitating the adhesion, survival, and 
      differentiation of NSCs. The NSCs in the defined medium alone group showed poor 
      survival and a very low level of neuronal differentiation and proliferation 
      abilities than above three groups. This study suggested that the BDNF-loaded 
      plasma-collagen matrix may provide a promising means to resolve either the poor 
      survival and insufficient neuronal differentiation of transplanted exogenous 
      NSCs, or stimulating the intrinsic NSCs to proliferate and differentiate into 
      neurons so as to repair the injured adult CNS.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Yang, Zhaoyang
AU  - Yang Z
AD  - Beijing Institute for Neuroscience, Capital Medical University, Beijing 100069, 
      China.
FAU - Qiao, Hui
AU  - Qiao H
FAU - Sun, Zhiwei
AU  - Sun Z
FAU - Li, Xiaoguang
AU  - Li X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Differentiation/drug effects/genetics
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects/genetics
MH  - Coculture Techniques
MH  - Collagen/*pharmacology
MH  - *Drug Delivery Systems
MH  - Gene Expression Regulation/drug effects
MH  - Kinetics
MH  - Neural Stem Cells/*cytology/drug effects/metabolism
MH  - Phenotype
MH  - Plasma/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/metabolism
EDAT- 2012/10/24 06:00
MHDA- 2013/05/29 06:00
CRDT- 2012/10/24 06:00
PHST- 2012/02/18 00:00 [received]
PHST- 2012/06/24 00:00 [revised]
PHST- 2012/06/26 00:00 [accepted]
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/05/29 06:00 [medline]
AID - 10.1002/jbm.a.34331 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2013 Feb;101(2):599-606. doi: 10.1002/jbm.a.34331. Epub 
      2012 Oct 23.