PMID- 23090945
OWN - NLM
STAT- MEDLINE
DCOM- 20130613
LR  - 20211021
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Print)
IS  - 0022-3751 (Linking)
VI  - 591
IP  - 1
DP  - 2013 Jan 1
TI  - Regulation of synaptic transmission at the photoreceptor terminal: a novel role 
      for the cation-chloride co-transporter NKCC1.
PG  - 133-47
LID - 10.1113/jphysiol.2012.241042 [doi]
AB  - The Na(+)-K(+)-2Cl(-) co-transporter type 1 (NKCC1) is localized primarily 
      throughout the outer plexiform layer (OPL) of the distal retina, a synaptic 
      lamina that is comprised of the axon terminals of photoreceptors and the 
      dendrites of horizontal and bipolar cells. Although known to play a key role in 
      development, signal transmission and the gating of sensory signals in other 
      regions of the retina and in the CNS, the contribution of NKCC1 to synaptic 
      transmission within the OPL is largely unknown. In the present study, we 
      investigated the function of NKCC1 at the photoreceptor-horizontal cell synapse 
      by recording the electrical responses of photoreceptors and horizontal cells 
      before and after blocking the activity of the transporter with bumetanide (BMN). 
      Because NKCC1 co-transports 1 Na(+), 1 K(+) and 2 Cl(-), it is electroneutral and 
      its activation had little effect on membrane conductance. However, recordings 
      from postsynaptic horizontal cells revealed that inhibiting NKCC1 with BMN 
      greatly increased glutamate release from both rod and cone terminals. In 
      addition, we found that NKCC1 directly regulates Ca(2+)-dependent exocytosis at 
      the photoreceptor synapse, raising the possibility that NKCC1 serves to suppress 
      bulk release of glutamate vesicles from photoreceptor terminals in the dark and 
      at light offset. Interestingly, NKCC1 gene and protein expressions were 
      upregulated by light, which we attribute to the light-induced release of dopamine 
      acting on D1-like receptors. In sum, our study reveals a new role for NKCC1 in 
      the regulation of synaptic transmission in photoreceptors.
FAU - Shen, Wen
AU  - Shen W
AD  - Department of Biomedical Science, Charles E Schmidt College of Medicine, Florida 
      Atlantic University, 777 Glades Road, Boca Raton, FL 33431, USA. wshen@fau.edu
FAU - Purpura, Lauren A
AU  - Purpura LA
FAU - Li, Baoqin
AU  - Li B
FAU - Nan, Changlong
AU  - Nan C
FAU - Chang, Irene J
AU  - Chang IJ
FAU - Ripps, Harris
AU  - Ripps H
LA  - eng
GR  - R01 EY014161/EY/NEI NIH HHS/United States
GR  - EY 14161/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121022
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 0 (Solute Carrier Family 12, Member 2)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - SY7Q814VUP (Calcium)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Ambystoma
MH  - Animals
MH  - Bumetanide/pharmacology
MH  - Calcium/physiology
MH  - Dopamine/physiology
MH  - Photoreceptor Cells/*physiology
MH  - Sodium-Potassium-Chloride Symporters/*physiology
MH  - Solute Carrier Family 12, Member 2
MH  - Synaptic Transmission/*physiology
PMC - PMC3630777
EDAT- 2012/10/24 06:00
MHDA- 2013/06/14 06:00
PMCR- 2014/01/01
CRDT- 2012/10/24 06:00
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/06/14 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - jphysiol.2012.241042 [pii]
AID - 10.1113/jphysiol.2012.241042 [doi]
PST - ppublish
SO  - J Physiol. 2013 Jan 1;591(1):133-47. doi: 10.1113/jphysiol.2012.241042. Epub 2012 
      Oct 22.