PMID- 2309100
OWN - NLM
STAT- MEDLINE
DCOM- 19900405
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 31
IP  - 2
DP  - 1990 Feb
TI  - Effects of heat shock on cytolysis mediated by NK cells, LAK cells, activated 
      monocytes and TNFs alpha and beta.
PG  - 175-82
AB  - We have recently shown that a heat treatment of a murine target cell line, WEHI 
      164, induces resistance to lysis mediated by tumour necrosis factors alpha 
      (TNF-alpha) and beta (TNF-beta). In the present study the effect of the heat 
      shock of target cells on cytotoxicity mediated by natural killer cells (NK 
      cells), lymphocyte-activated killer cells (LAK cells), activated monocytes, 
      TNF-alpha, and TNF-beta was investigated. First, WEHI 164 cell line and six human 
      cell lines (ME 180, K 562, U 937, HeLa, MCF7, and SK-OV 3) were screened for 
      their sensitivity to different forms of lysis, and then sensitive cell lines were 
      heat-treated. Pretreatment of target cells at 42 degrees C for 45-60 min also 
      rendered human target cell lines more resistant to lysis by rTNFs, and the 
      acquired resistance was accompanied by an increased resistance to activated 
      monocytes, but not to NK cells or LAK cells. Thus, the heat-induced resistance 
      mechanisms capable of protecting target cells from lysis by rTNFs and by 
      activated monocytes do not elicit resistance to lysis by NK cells and LAK cells, 
      supporting the hypothesis that mediators other than TNFs are involved in NK cell- 
      and LAK cell-mediated killing.
FAU - Jaattela, M
AU  - Jaattela M
AD  - Department of Pathology, University of Helsinki, Finland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic
MH  - *Hot Temperature
MH  - Humans
MH  - Killer Cells, Lymphokine-Activated/*physiology
MH  - Killer Cells, Natural/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphotoxin-alpha/*physiology
MH  - Monocytes/*physiology
MH  - Recombinant Proteins
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 10.1111/j.1365-3083.1990.tb02757.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1990 Feb;31(2):175-82. doi: 10.1111/j.1365-3083.1990.tb02757.x.