PMID- 23091648
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Identification of differentially-expressed genes associated with pistil abortion 
      in Japanese apricot by genome-wide transcriptional analysis.
PG  - e47810
LID - 10.1371/journal.pone.0047810 [doi]
LID - e47810
AB  - The phenomenon of pistil abortion widely occurs in Japanese apricot, and 
      imperfect flowers with pistil abortion seriously decrease the yield in 
      production. Although transcriptome analyses have been extensively studied in the 
      past, a systematic study of differential gene expression has not been performed 
      in Japanese apricot. To investigate genes related to the pistil development of 
      Japanese apricot, high-throughput sequencing technology (Illumina) was employed 
      to survey gene expression profiles from perfect and imperfect Japanese apricot 
      flower buds. 3,476,249 and 3,580,677 tags were sequenced from two libraries 
      constructed from perfect and imperfect flower buds of Japanese apricot, 
      respectively. There were 689 significant differentially-expressed genes between 
      the two libraries. GO annotation revealed that highly ranked genes were those 
      implicated in small molecule metabolism, cellular component organisation or 
      biogenesis at the cellular level and fatty acid metabolism. According to the 
      results, we assumed that late embryogenesis abundant protein (LEA), Dicer-like 3 
      (DCL3) Xyloglucan endotransglucosylase/hydrolase 2 (XTH2), Pectin lyase-like 
      superfamily protein (PPME1), Lipid transfer protein 3 (LTP3), Fatty acid 
      biosynthesis 1 (FAB1) and Fatty acid desaturase 5 (FAD5) might have relationships 
      with the pistil abortion in Japanese apricot. The expression patterns of 36 
      differentially expressed genes were confirmed by real-time (RT)-PCR. This is the 
      first report of the Illumina RNA-seq technique being used for the analysis of 
      differentially-expressed gene profiles related to pistil abortion that both 
      computationally and experimentally provides valuable information for the further 
      functional characterisation of genes associated with pistil development in woody 
      plants.
FAU - Shi, Ting
AU  - Shi T
AD  - College of Horticulture, Nanjing Agricultural University, Nanjing, People's 
      Republic China.
FAU - Gao, Zhihong
AU  - Gao Z
FAU - Wang, Liangju
AU  - Wang L
FAU - Zhang, Zhen
AU  - Zhang Z
FAU - Zhuang, Weibing
AU  - Zhuang W
FAU - Sun, Hailong
AU  - Sun H
FAU - Zhong, Wenjun
AU  - Zhong W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121016
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Expressed Sequence Tags
MH  - Flowers/*genetics
MH  - *Gene Expression Regulation, Plant
MH  - Molecular Sequence Annotation
MH  - Prunus/*genetics
MH  - Signal Transduction
MH  - *Transcriptome
PMC - PMC3472986
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/10/24 06:00
MHDA- 2013/03/30 06:00
PMCR- 2012/10/16
CRDT- 2012/10/24 06:00
PHST- 2012/06/19 00:00 [received]
PHST- 2012/09/17 00:00 [accepted]
PHST- 2012/10/24 06:00 [entrez]
PHST- 2012/10/24 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
PHST- 2012/10/16 00:00 [pmc-release]
AID - PONE-D-12-17797 [pii]
AID - 10.1371/journal.pone.0047810 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e47810. doi: 10.1371/journal.pone.0047810. Epub 2012 Oct 16.