PMID- 23093651
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20221207
IS  - 1468-3296 (Electronic)
IS  - 0040-6376 (Linking)
VI  - 68
IP  - 2
DP  - 2013 Feb
TI  - Airway inflammation in patients with chronic non-asthmatic cough.
PG  - 125-30
LID - 10.1136/thoraxjnl-2012-201895 [doi]
AB  - INTRODUCTION: Chronic non-asthmatic cough (CC) is a clinical challenge and 
      underlying pathophysiological mechanisms remain still not completely understood. 
      One of the most common comorbidities in CC is gastro-oesophageal reflux disease 
      (GORD). Airway epithelium damage can contribute to airway inflammation in CC. 
      AIMS: We studied airway inflammation in patients with CC compared to healthy 
      controls. Patients with GORD were treated with proton pump inhibitors (PPI) and 
      cough response to PPI was evaluated. PATIENTS AND METHODS: Sputum was induced in 
      41 adults with CC and 20 healthy non-smokers who were age and sex matched. We 
      compared sputum differential cell count by cytospin and cytokine and chemokine 
      production at the mRNA and/or protein levels by real-time (RT)-PCR and cytokine 
      bead array (CBA), between patients with CC and healthy subjects. Furthermore we 
      studied airway inflammation in patients with different comorbidities. RESULTS: No 
      differences in sputum differential cell counts were observed between patients 
      with CC and healthy subjects. Sputum monocyte chemoattractant protein-1 (MCP-1) 
      protein levels were significantly higher in patients when compared to controls. 
      Thymic stromal lymphopoietin (TSLP) mRNA was significantly more often expressed 
      in sputum of patients with CC than from healthy controls. Sputum transforming 
      growth factor (TGF)-beta levels did not differ between patients and controls, but 
      were significantly lower in the PPI responders compared to the non-responders; 
      p=0.047. There is no evidence for impaired T helper cell (Th)1/Th2/Th17 balance 
      in CC. Patients with reflux oesophagitis (RO) have significantly more sputum 
      eosinophils than patients without RO. CONCLUSIONS: CC is a condition presenting 
      with different disease phenotypes. High sputum MCP-1 levels are present in a 
      large group of patients with CC and a majority of these patients with CC have 
      increased sputum TSLP levels, most likely produced by damaged airway epithelial 
      cells.
FAU - Grabowski, Marcin
AU  - Grabowski M
AD  - Department of Physiology, Wroclaw Medical University, Wroclaw, Poland. 
      marcingrabow@o2.pl
FAU - Seys, Sven
AU  - Seys S
FAU - Decraene, Ann
AU  - Decraene A
FAU - Kasran, Ahmad
AU  - Kasran A
FAU - Dilissen, Ellen
AU  - Dilissen E
FAU - Barg, Wojciech
AU  - Barg W
FAU - Medrala, Wojciech
AU  - Medrala W
FAU - Dupont, Lieven J
AU  - Dupont LJ
FAU - Panaszek, Bernard
AU  - Panaszek B
FAU - Bullens, Dominique M A
AU  - Bullens DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - England
TA  - Thorax
JT  - Thorax
JID - 0417353
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Proton Pump Inhibitors)
RN  - 0 (Transforming Growth Factor beta)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Adult
MH  - Bronchi/pathology
MH  - Bronchitis/*epidemiology/pathology
MH  - Cell Count
MH  - Chemokine CCL2/analysis
MH  - Comorbidity
MH  - Cough/*epidemiology/genetics/pathology
MH  - Cytokines/analysis
MH  - Female
MH  - Gastroesophageal Reflux/drug therapy/epidemiology
MH  - Humans
MH  - Inflammation/metabolism
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Proton Pump Inhibitors/therapeutic use
MH  - Sputum/cytology/metabolism
MH  - Transforming Growth Factor beta/analysis
MH  - Thymic Stromal Lymphopoietin
EDAT- 2012/10/25 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/10/25 06:00
PHST- 2012/10/25 06:00 [entrez]
PHST- 2012/10/25 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - thoraxjnl-2012-201895 [pii]
AID - 10.1136/thoraxjnl-2012-201895 [doi]
PST - ppublish
SO  - Thorax. 2013 Feb;68(2):125-30. doi: 10.1136/thoraxjnl-2012-201895. Epub 2012 Oct 
      23.