PMID- 23095308
OWN - NLM
STAT- MEDLINE
DCOM- 20130718
LR  - 20121213
IS  - 1423-0216 (Electronic)
IS  - 1021-7401 (Linking)
VI  - 20
IP  - 1
DP  - 2013
TI  - Lymphocyte-derived catecholamines induce a shift of Th1/Th2 balance toward Th2 
      polarization.
PG  - 1-8
LID - 10.1159/000343099 [doi]
AB  - AIMS: Our previous work has shown that lymphocytes synthesize and secrete 
      catecholamines (CAs), which regulate lymphocyte proliferation and apoptosis. In 
      the present study, we explored the effect of the lymphocyte-derived CAs on 
      differentiation and function of T helper (Th) cells. METHODS: Lymphocytes were 
      separated from the mesenteric lymph nodes of mice and stimulated by concanavalin 
      A (Con A). These cells were treated with alpha-methyl-p- tyrosine (alpha-MT), an 
      inhibitor of tyrosine hydroxylase (TH) that is a rate-limiting enzyme for 
      synthesis of CAs, and pargyline, an inhibitor of monoamine oxidase that degrades 
      CAs. RESULTS: Treatment of Con A-stimulated lymphocytes with alpha-MT (10(-6) M) 
      reduced CAs both in the cultured lymphocytes and in the culture supernatants. 
      Simultaneously, alpha-MT upregulated expression of mRNAs and proteins of T-box 
      expressed in T cells (T-bet) and interferon-gamma (IFN-gamma) but downregulated 
      expression of mRNAs and proteins of GATA binding protein 3 (GATA-3) and 
      interleukin-4 (IL-4) in Con A-activated lymphocytes. In contrast, pargyline 
      (10(-6) M) increased intracellular and supernatant CA contents in Con A-activated 
      lymphocytes. Meanwhile, the treatment with pargyline downregulated expression of 
      T-bet and IFN-gamma but upregulated expression of GATA-3 and IL-4 in these 
      lymphocytes. CONCLUSION: CAs synthesized and secreted by lymphocytes regulate 
      differentiation and function of Th cells, with an effect facilitating the shift 
      of Th1/Th2 balance toward Th2 polarization.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Huang, Hui-Wei
AU  - Huang HW
AD  - Department of Physiology, School of Medicine, Nantong University, Nantong, PR 
      China.
FAU - Tang, Jian-Lei
AU  - Tang JL
FAU - Han, Xin-Hua
AU  - Han XH
FAU - Peng, Yu-Ping
AU  - Peng YP
FAU - Qiu, Yi-Hua
AU  - Qiu YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Catecholamines)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Catecholamines/*metabolism
MH  - Cell Differentiation/immunology
MH  - *Cell Polarity
MH  - Chromatography, High Pressure Liquid
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Th1 Cells/*cytology/immunology/*metabolism
MH  - *Th1-Th2 Balance
MH  - Th2 Cells/*cytology/immunology/*metabolism
EDAT- 2012/10/26 06:00
MHDA- 2013/07/19 06:00
CRDT- 2012/10/26 06:00
PHST- 2012/06/01 00:00 [received]
PHST- 2012/08/28 00:00 [accepted]
PHST- 2012/10/26 06:00 [entrez]
PHST- 2012/10/26 06:00 [pubmed]
PHST- 2013/07/19 06:00 [medline]
AID - 000343099 [pii]
AID - 10.1159/000343099 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 2013;20(1):1-8. doi: 10.1159/000343099. Epub 2012 Oct 23.