PMID- 23097308
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20240109
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 31
IP  - 5
DP  - 2013 Jul
TI  - Effect of storage time on haematological parameters in mullet, Mugil cephalus.
PG  - 412-6
LID - 10.1002/cbf.2915 [doi]
AB  - The aim of the present study was to assess the effect of storage time at +4  degrees C on 
      red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell 
      count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean 
      corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) 
      in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 
      ), all samples were analyzed using both the manual and automatic method. To test 
      the validation of the automatic method, a paired t-test was applied, and no 
      statistical difference was observed. The samples were successively divided into 
      four different aliquots and stored at +4  degrees C to assess the haematological 
      parameters using the automatic method. The first aliquot was refrigerated for 6 
      h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. 
      One-way repeated-measures ANOVA showed a significant effect of storage time 
      (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that 
      haematological parameters can be assessed within 6 h from blood collection when 
      samples are stored at +4  degrees C because long-term storage modifies the results of the 
      analyses. Further studies on these parameters could be still needed in various 
      fish species to validate an appropriate method for haematological analysis useful 
      not only for the evaluation of the health status of animal living in captivity 
      and in aquaculture but also to have reliability environmental haematological 
      biomarkers.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Faggio, C
AU  - Faggio C
AD  - Faculty of Science, Department of Life Sciences M. Malpighi Section of General 
      Physiology, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 S. 
      Agata, 98166 Messina, Italy.
FAU - Casella, S
AU  - Casella S
FAU - Arfuso, F
AU  - Arfuso F
FAU - Marafioti, S
AU  - Marafioti S
FAU - Piccione, G
AU  - Piccione G
FAU - Fazio, F
AU  - Fazio F
LA  - eng
PT  - Journal Article
DEP - 20121024
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Automation, Laboratory
MH  - Blood Platelets/*chemistry/cytology
MH  - Erythrocyte Count
MH  - Erythrocyte Indices
MH  - Erythrocytes/*chemistry/cytology
MH  - Hematocrit
MH  - Leukocyte Count
MH  - Leukocytes/*chemistry/cytology
MH  - Male
MH  - Platelet Count
MH  - Refrigeration/*standards
MH  - Reproducibility of Results
MH  - Smegmamorpha/*blood
MH  - Specimen Handling/*standards
OTO - NOTNLM
OT  - Mugil cephalus
OT  - haematological automatic analysis
OT  - haematological parameters
OT  - storage time
OT  - teleost
EDAT- 2012/10/26 06:00
MHDA- 2014/01/22 06:00
CRDT- 2012/10/26 06:00
PHST- 2012/06/20 00:00 [received]
PHST- 2012/09/06 00:00 [revised]
PHST- 2012/09/12 00:00 [accepted]
PHST- 2012/10/26 06:00 [entrez]
PHST- 2012/10/26 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
AID - 10.1002/cbf.2915 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2013 Jul;31(5):412-6. doi: 10.1002/cbf.2915. Epub 2012 Oct 
      24.