PMID- 23098478
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20190606
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 13
IP  - 8
DP  - 2012
TI  - Combined study of cytogenetics and fluorescence in situ hybridization (FISH) 
      analysis in childhood acute lymphoblastic leukemia (ALL) in a tertiary cancer 
      centre in South India.
PG  - 3825-7
AB  - FISH is one of the most sensitive molecular methods to detect genetic 
      abnormalities with DNA probes. When cytogenetic studies are normal or 
      insufficient, FISH may detect cryptic rearrangements, rare or slowly 
      proliferative abnormal populations in non-mitotic cells. We cytogenetically 
      evaluated 70 childhood ALL - 67.1% were found to have an abnormal karyotype. The 
      23 patients (32.9%) with a normal karyotype were analyzed by FISH applying two 
      probes; TEL/AML1 and MYB which detect cryptic rearrangements of t(12;21)(p13;q22) 
      and deletion of (6q) respectively, associated with a good prognosis. Out of 23 
      patients, one was positive for t(12;21)(p13;q22) (4.3%). None of our patients 
      were positive for MYB del(6q). Two patients showed an extra signal for MYB on 
      chromosomes other than 6 (8.6 %) indicating amplification or duplication. 
      Findings were compared with the available literature. Our study clearly indicated 
      the integrated FISH screening method to increase the abnormality detection rate 
      in a narrow range. FISH is less useful for diagnostic study of patients with 
      suspected del(6q) but it helps in detecting known cryptic rearrangements as well 
      as identification of new abnormalities(translocation , duplication and 
      amplification) at the gene level.
FAU - Mazloumi, Seyed Hashem Mir
AU  - Mazloumi SH
AD  - Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore, India.
FAU - Madhumathi, D S
AU  - Madhumathi DS
FAU - Appaji, L
AU  - Appaji L
FAU - Prasannakumari
AU  - Prasannakumari
LA  - eng
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins c-myb)
RN  - 0 (TEL-AML1 fusion protein)
SB  - IM
MH  - Child
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Core Binding Factor Alpha 2 Subunit/*genetics
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - India
MH  - Male
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-myb/genetics
MH  - Tertiary Care Centers
EDAT- 2012/10/27 06:00
MHDA- 2013/04/20 06:00
CRDT- 2012/10/27 06:00
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - 10.7314/apjcp.2012.13.8.3825 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2012;13(8):3825-7. doi: 10.7314/apjcp.2012.13.8.3825.